Detailed information NTG0990267

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY12_RS21855
• Genome accession: NZ_CP153038
• Coordinates: 4525680..4526480 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-220
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4520680..4531480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY12_RS21820 (AAHY12_21780)	gspH	4520833..4521348 (+)	516	WP_046882113.1	type II secretion system minor pseudopilin GspH	-
AAHY12_RS21825 (AAHY12_21785)	gspI	4521345..4521710 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY12_RS21830 (AAHY12_21790)	gspJ	4521707..4522303 (+)	597	WP_072121920.1	type II secretion system minor pseudopilin GspJ	-
AAHY12_RS21835 (AAHY12_21795)	gspK	4522300..4523280 (+)	981	WP_374741257.1	type II secretion system minor pseudopilin GspK	-
AAHY12_RS21840 (AAHY12_21800)	gspL	4523273..4524469 (+)	1197	WP_046620843.1	type II secretion system protein GspL	-
AAHY12_RS21845 (AAHY12_21805)	-	4524462..4524947 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHY12_RS21850 (AAHY12_21810)	-	4524937..4525683 (+)	747	WP_049157077.1	type II secretion system protein N	-
AAHY12_RS21855 (AAHY12_21815)	pilD	4525680..4526480 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHY12_RS21860 (AAHY12_21820)	hrpB	4526527..4528956 (-)	2430	WP_101880591.1	ATP-dependent helicase HrpB	-
AAHY12_RS21865 (AAHY12_21825)	thpR	4529029..4529565 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY12_RS21870 (AAHY12_21830)	sfsA	4529565..4530281 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHY12_RS21875 (AAHY12_21835)	dksA	4530444..4530899 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990267 AAHY12_RS21855 WP_008807384.1 4525680..4526480(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-220]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990267 AAHY12_RS21855 WP_008807384.1 4525680..4526480(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-220]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425