Detailed information NTG0990244

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY18_RS21125
• Genome accession: NZ_CP153019
• Coordinates: 4360389..4361189 (+)
• Length: 266 a.a.
• NCBI ID: WP_023292017.1
• Uniprot ID: A0A483KIE1
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-262
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4355389..4366189

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY18_RS21090 (AAHY18_21040)	gspH	4355542..4356057 (+)	516	WP_131096195.1	type II secretion system minor pseudopilin GspH	-
AAHY18_RS21095 (AAHY18_21045)	gspI	4356054..4356419 (+)	366	WP_114465183.1	type II secretion system minor pseudopilin GspI	-
AAHY18_RS21100 (AAHY18_21050)	gspJ	4356416..4357012 (+)	597	WP_048323018.1	type II secretion system minor pseudopilin GspJ	-
AAHY18_RS21105 (AAHY18_21055)	gspK	4357009..4357989 (+)	981	WP_374749039.1	type II secretion system minor pseudopilin GspK	-
AAHY18_RS21110 (AAHY18_21060)	gspL	4357982..4359178 (+)	1197	WP_101989312.1	type II secretion system protein GspL	-
AAHY18_RS21115 (AAHY18_21065)	-	4359171..4359656 (+)	486	WP_023292019.1	type II secretion system protein M	-
AAHY18_RS21120 (AAHY18_21070)	-	4359646..4360374 (+)	729	WP_112201876.1	type II secretion system protein N	-
AAHY18_RS21125 (AAHY18_21075)	pilD	4360389..4361189 (+)	801	WP_023292017.1	A24 family peptidase	Machinery gene
AAHY18_RS21130 (AAHY18_21080)	hrpB	4361237..4363666 (-)	2430	WP_348849563.1	ATP-dependent helicase HrpB	-
AAHY18_RS21135 (AAHY18_21085)	thpR	4363739..4364275 (+)	537	WP_348849564.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY18_RS21140 (AAHY18_21090)	sfsA	4364275..4364991 (+)	717	WP_023292014.1	DNA/RNA nuclease SfsA	-
AAHY18_RS21145 (AAHY18_21095)	dksA	4365154..4365609 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29274.82 Da
• Isoelectric Point: 8.8894

>NTDB_id=990244 AAHY18_RS21125 WP_023292017.1 4360389..4361189(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-262]
MTTLAALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDEPITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQARISWSYPLTELATGLLFILAGALLAPGLALAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQALPQVLLLASASGLAWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGVFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990244 AAHY18_RS21125 WP_023292017.1 4360389..4361189(+) (pilD) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain T7-262]
ATGACGACATTAGCCGCATTATCGCTGCATTTTCCCCTTGTCTGGTATGGTTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTACCGTCTGCCGCGCATGCTGACCCAAACCGCGGACGACGAGCCGATAACCCTCAGCA
CGCCGGGCTCATCCTGCCCGCAGTGTCGCCAGCCGATAGCCTGGCGGGACAATATCCCGCTGCTCAGCTTCCTCTGGCTA
GGCCGTCGCGCCCGCTGCTGTCAGGCGCGCATCTCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGGCGCTGGCGGGCGGCCTGGTGCTGCTCTCCTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAACTGCTGCCCGATCGCCTGACCCTGCCCCTGCTGTGGGCCGGACTGCTGTTTAATCTTAAC
GAGGTGTACATTGCGCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGCAAAGAGGCCCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGCGCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCAGCGGGCTGGCGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGAGGGGGCGTTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A483KIE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425