Detailed information NTG0990173

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHW07_RS21610
• Genome accession: NZ_CP152988
• Coordinates: 4460546..4461346 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain NK_H1_059
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4455546..4466346

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHW07_RS21575 (AAHW07_21560)	gspH	4455699..4456214 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHW07_RS21580 (AAHW07_21565)	gspI	4456211..4456576 (+)	366	WP_264969680.1	type II secretion system minor pseudopilin GspI	-
AAHW07_RS21585 (AAHW07_21570)	gspJ	4456573..4457169 (+)	597	WP_032731630.1	type II secretion system minor pseudopilin GspJ	-
AAHW07_RS21590 (AAHW07_21575)	gspK	4457166..4458146 (+)	981	WP_077264701.1	type II secretion system minor pseudopilin GspK	-
AAHW07_RS21595 (AAHW07_21580)	gspL	4458139..4459335 (+)	1197	WP_072125473.1	type II secretion system protein GspL	-
AAHW07_RS21600 (AAHW07_21585)	-	4459328..4459813 (+)	486	WP_008807382.1	type II secretion system protein M	-
AAHW07_RS21605 (AAHW07_21590)	-	4459803..4460549 (+)	747	WP_023322077.1	type II secretion system protein N	-
AAHW07_RS21610 (AAHW07_21595)	pilD	4460546..4461346 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHW07_RS21615 (AAHW07_21600)	hrpB	4461393..4463822 (-)	2430	WP_086892927.1	ATP-dependent helicase HrpB	-
AAHW07_RS21620 (AAHW07_21605)	thpR	4463895..4464431 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHW07_RS21625 (AAHW07_21610)	sfsA	4464431..4465147 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHW07_RS21630 (AAHW07_21615)	dksA	4465310..4465765 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990173 AAHW07_RS21610 WP_008807384.1 4460546..4461346(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H1_059]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990173 AAHW07_RS21610 WP_008807384.1 4460546..4461346(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H1_059]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425