Detailed information NTG0990103

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHW31_RS22430
• Genome accession: NZ_CP152915
• Coordinates: 4622466..4623266 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain NK_H12_100
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4617466..4628266

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHW31_RS22395 (AAHW31_22380)	gspH	4617619..4618134 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHW31_RS22400 (AAHW31_22385)	gspI	4618131..4618496 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHW31_RS22405 (AAHW31_22390)	gspJ	4618493..4619089 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
AAHW31_RS22410 (AAHW31_22395)	gspK	4619086..4620066 (+)	981	WP_077257383.1	type II secretion system minor pseudopilin GspK	-
AAHW31_RS22415 (AAHW31_22400)	gspL	4620059..4621255 (+)	1197	WP_044613515.1	type II secretion system protein GspL	-
AAHW31_RS22420 (AAHW31_22405)	-	4621248..4621733 (+)	486	WP_044613514.1	type II secretion system protein M	-
AAHW31_RS22425 (AAHW31_22410)	-	4621723..4622469 (+)	747	WP_374447645.1	type II secretion system protein N	-
AAHW31_RS22430 (AAHW31_22415)	pilD	4622466..4623266 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHW31_RS22435 (AAHW31_22420)	hrpB	4623313..4625742 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHW31_RS22440 (AAHW31_22425)	thpR	4625815..4626351 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHW31_RS22445 (AAHW31_22430)	sfsA	4626351..4627067 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
AAHW31_RS22450 (AAHW31_22435)	dksA	4627230..4627685 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990103 AAHW31_RS22430 WP_008807384.1 4622466..4623266(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H12_100]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990103 AAHW31_RS22430 WP_008807384.1 4622466..4623266(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H12_100]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425