Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   EC958_RS22600 Genome accession   NZ_HG941718
Coordinates   4496663..4498861 (-) Length   732 a.a.
NCBI ID   WP_001296625.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131 strain EC958     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4491663..4503861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC958_RS22580 (EC958_4416) hslU 4492467..4493798 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  EC958_RS22585 (EC958_4417) hslV 4493808..4494338 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  EC958_RS22590 (EC958_4418) ftsN 4494431..4495390 (-) 960 WP_000068834.1 cell division protein FtsN -
  EC958_RS22595 (EC958_4419) cytR 4495482..4496507 (-) 1026 WP_000644908.1 DNA-binding transcriptional regulator CytR Regulator
  EC958_RS22600 (EC958_4420) priA 4496663..4498861 (-) 2199 WP_001296625.1 primosomal protein N' Machinery gene
  EC958_RS22605 (EC958_4421) rpmE 4499064..4499276 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  EC958_RS22610 (EC958_4422) yiiX 4499337..4499945 (-) 609 WP_000702314.1 YiiX family permuted papain-like enzyme -
  EC958_RS22615 (EC958_4423) metJ 4500005..4500322 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  EC958_RS22620 (EC958_4424) metB 4500599..4501759 (+) 1161 WP_000197203.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81669.91 Da        Isoelectric Point: 9.0002

>NTDB_id=989991 EC958_RS22600 WP_001296625.1 4496663..4498861(-) (priA) [Escherichia coli O25b:H4-ST131 strain EC958]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=989991 EC958_RS22600 WP_001296625.1 4496663..4498861(-) (priA) [Escherichia coli O25b:H4-ST131 strain EC958]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGAGTCGCGTGCGCGTGCCGTTTGGCAAACAACAGGAGCGCATCGGGGTTGTGGTGTCAGTGAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCAAACGCGCCTATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGATCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGGTTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCGGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGTTCCTACAAACAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTGATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGTGGCTGGATTGCCGAATGCCCGCGTTGCGATCACTACTACACGC
TGCATCAGGCACAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACACACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGCTGTTCCCCGGCGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCTCAGCTTTACACCCAGGTCGCTGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGACTTTGCCGAACAGGCGCTGGCTGAGCGACGGATGATGCAGCTGCCGCCATGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAGTTGCGTAATCTGATCCTCGCCAGCCCGCTTGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCCCCTAAGCGTGGTGGCCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGATTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGTAAA
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451