Detailed information NTG0989778

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHW99_RS22245
• Genome accession: NZ_CP152721
• Coordinates: 4544375..4545175 (+)
• Length: 266 a.a.
• NCBI ID: WP_374182981.1
• Uniprot ID: -
• Organism: Klebsiella variicola subsp. variicola strain NK_H21_067
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4539375..4550175

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHW99_RS22210 (AAHW99_22190)	gspH	4539528..4540043 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHW99_RS22215 (AAHW99_22195)	gspI	4540040..4540405 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHW99_RS22220 (AAHW99_22200)	gspJ	4540402..4540998 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
AAHW99_RS22225 (AAHW99_22205)	gspK	4540995..4541975 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
AAHW99_RS22230 (AAHW99_22210)	gspL	4541968..4543164 (+)	1197	WP_110209646.1	type II secretion system protein GspL	-
AAHW99_RS22235 (AAHW99_22215)	-	4543157..4543642 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHW99_RS22240 (AAHW99_22220)	-	4543632..4544378 (+)	747	WP_044613513.1	type II secretion system protein N	-
AAHW99_RS22245 (AAHW99_22225)	pilD	4544375..4545175 (+)	801	WP_374182981.1	A24 family peptidase	Machinery gene
AAHW99_RS22250 (AAHW99_22230)	hrpB	4545222..4547651 (-)	2430	WP_374182982.1	ATP-dependent helicase HrpB	-
AAHW99_RS22255 (AAHW99_22235)	thpR	4547724..4548260 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHW99_RS22260 (AAHW99_22240)	sfsA	4548260..4548976 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHW99_RS22265 (AAHW99_22245)	dksA	4549139..4549594 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29475.22 Da
• Isoelectric Point: 8.7151

>NTDB_id=989778 AAHW99_RS22245 WP_374182981.1 4544375..4545175(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H21_067]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATSLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=989778 AAHW99_RS22245 WP_374182981.1 4544375..4545175(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H21_067]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCAGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	42.912	98.12	0.421