Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V7S45_RS20800 Genome accession   NZ_CP152433
Coordinates   4048764..4049807 (-) Length   347 a.a.
NCBI ID   WP_089607630.1    Uniprot ID   -
Organism   Bacillus sp. BR3(2024)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4043764..4054807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7S45_RS20755 (V7S45_20760) - 4044197..4044397 (-) 201 WP_000106083.1 YqzE family protein -
  V7S45_RS20760 (V7S45_20765) - 4044436..4044933 (-) 498 WP_089607627.1 shikimate kinase -
  V7S45_RS20765 (V7S45_20770) - 4045049..4045699 (-) 651 WP_000183857.1 2OG-Fe(II) oxygenase -
  V7S45_RS20770 (V7S45_20775) comGG 4045874..4046245 (-) 372 WP_089607628.1 competence type IV pilus minor pilin ComGG -
  V7S45_RS20775 (V7S45_20780) comGF 4046242..4046712 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  V7S45_RS20780 (V7S45_20785) - 4046682..4046984 (-) 303 WP_000829470.1 hypothetical protein -
  V7S45_RS20785 (V7S45_20790) comGD 4046977..4047432 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  V7S45_RS20790 (V7S45_20795) comGC 4047429..4047728 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  V7S45_RS20795 (V7S45_20800) comGB 4047740..4048777 (-) 1038 WP_100249632.1 competence type IV pilus assembly protein ComGB -
  V7S45_RS20800 (V7S45_20805) comGA 4048764..4049807 (-) 1044 WP_089607630.1 competence type IV pilus ATPase ComGA Machinery gene
  V7S45_RS20805 (V7S45_20810) - 4050013..4050708 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  V7S45_RS20810 (V7S45_20815) - 4050834..4051076 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  V7S45_RS20815 (V7S45_20820) - 4051184..4052578 (+) 1395 WP_001094345.1 L-cystine transporter -
  V7S45_RS20820 (V7S45_20825) - 4052657..4053049 (-) 393 WP_000875394.1 hypothetical protein -
  V7S45_RS20825 (V7S45_20830) - 4053137..4053352 (-) 216 WP_001008327.1 DUF3912 family protein -
  V7S45_RS20830 (V7S45_20835) - 4053605..4053916 (+) 312 WP_001093237.1 hypothetical protein -
  V7S45_RS20835 (V7S45_20840) - 4053951..4054439 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39081.47 Da        Isoelectric Point: 8.7212

>NTDB_id=989380 V7S45_RS20800 WP_089607630.1 4048764..4049807(-) (comGA) [Bacillus sp. BR3(2024)]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQDIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=989380 V7S45_RS20800 WP_089607630.1 4048764..4049807(-) (comGA) [Bacillus sp. BR3(2024)]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGATATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment