Detailed information NTG0989311

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: AAG068_RS05820
• Genome accession: NZ_CP152427
• Coordinates: 1129995..1133720 (+)
• Length: 1241 a.a.
• NCBI ID: WP_342718519.1
• Uniprot ID: -
• Organism: Bacillus paramycoides strain JYZ-SD5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1124995..1138720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAG068_RS05805 (AAG068_05805)	-	1125153..1125746 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
AAG068_RS05810 (AAG068_05810)	lepB	1125803..1126366 (+)	564	WP_342718517.1	signal peptidase I	-
AAG068_RS05815 (AAG068_05815)	addB	1126483..1129998 (+)	3516	WP_342718518.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
AAG068_RS05820 (AAG068_05820)	addA	1129995..1133720 (+)	3726	WP_342718519.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
AAG068_RS05825 (AAG068_05825)	-	1133733..1134020 (+)	288	WP_342718520.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
AAG068_RS05830 (AAG068_05830)	gerPF	1134100..1134315 (-)	216	WP_001141566.1	spore germination protein GerPF	-
AAG068_RS05835 (AAG068_05835)	-	1134357..1134743 (-)	387	WP_342718521.1	spore germination protein GerPE	-
AAG068_RS05840 (AAG068_05840)	gerPD	1134759..1134953 (-)	195	WP_342718522.1	spore germination protein GerPD	-
AAG068_RS05845 (AAG068_05845)	gerPC	1134960..1135574 (-)	615	WP_342718523.1	spore germination protein GerPC	-
AAG068_RS05850 (AAG068_05850)	gerPB	1135643..1135849 (-)	207	WP_087957704.1	spore germination protein GerPB	-
AAG068_RS05855 (AAG068_05855)	gerPA	1135864..1136085 (-)	222	WP_001111188.1	spore germination protein GerPA	-
AAG068_RS05860 (AAG068_05860)	-	1136182..1136361 (-)	180	WP_000462846.1	aspartyl-phosphate phosphatase Spo0E family protein	-
AAG068_RS05865 (AAG068_05865)	-	1136608..1137507 (+)	900	WP_342718525.1	fumarylacetoacetate hydrolase family protein	-
AAG068_RS05870 (AAG068_05870)	-	1137543..1137827 (-)	285	WP_342718526.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142686.87 Da
• Isoelectric Point: 4.8494

>NTDB_id=989311 AAG068_RS05820 WP_342718519.1 1129995..1133720(+) (addA) [Bacillus paramycoides strain JYZ-SD5]
MIENWPKKPEGSQWTDDQWKAVVANGRDVLVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVPDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGLPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRILYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQINGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=989311 AAG068_RS05820 WP_342718519.1 1129995..1133720(+) (addA) [Bacillus paramycoides strain JYZ-SD5]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATGTTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCATTAGAAAAAGTATTAATTGATGAGCCAGGCTCGCAGCACATTAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTCGTTGACCGTTATACAAGTGACCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACGGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACATTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCCGCTCGTGAATCGTGGACAAGCGTATATGAAGCGATGCAAAACGTATCATGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTAGCGCTCCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAATTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTAGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGCTTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTAGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTACGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACGGAAGAAGAAGTGCCAGACGGTGAAGAAGGTGCCGAAGTAGAAAAAGCTCAGCTTGAAGCTCGACTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGTTGCAAGGACTTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCAATTGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAAAAAGGCTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAACAAGAAC
TACACGATAAATTAGAGTGGTTCTATAACTTACTACAAGGATGGCGTGAATTCGCACGCCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
ACGCATACTATATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCAAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGACTAGAGTTCCCAGTCGTATTCGTAGCTGGGTTAGGTCGCCGTTTTAACACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACTCGT
GCAAAAGAGAAGCTAATTTTAATTGGAACAGTGAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAGCATTCCAGGTGAAATTTATGGATACGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTATTAGAAGCGCTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTATGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCGCCAATTAAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTACGCTGAAAGAGG
TACGGCAGTTCACGCTGTCATGCAGCATGTCGATTTAAAGAAGCCGATTACAGAAGAAGTGATTCGGGAACAAATTAATG
GCATGGTCAATAAAGAGTTATTAACATTCGAACAAGCAGAAGAAATAGCAATCGAAAAAGTCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTACTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGAAAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATTGACTGTATGATTGAAGAAGAAGACG
GTATCACGTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAGGCGAAACCAATTTTA
GAAGATCGATATAAAGTGCAGCTTTCTTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAACCATGTTGTAAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534