Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAG068_RS05815 Genome accession   NZ_CP152427
Coordinates   1126483..1129998 (+) Length   1171 a.a.
NCBI ID   WP_342718518.1    Uniprot ID   -
Organism   Bacillus paramycoides strain JYZ-SD5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1121483..1134998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG068_RS05780 (AAG068_05780) cspA 1122634..1122837 (+) 204 WP_000301523.1 RNA chaperone/antiterminator CspA -
  AAG068_RS05785 (AAG068_05785) - 1122953..1123105 (+) 153 Protein_1042 IS5/IS1182 family transposase -
  AAG068_RS05790 (AAG068_05790) - 1123272..1123484 (-) 213 WP_342718510.1 hypothetical protein -
  AAG068_RS05795 (AAG068_05795) - 1123793..1123978 (-) 186 WP_342718511.1 hypothetical protein -
  AAG068_RS05800 (AAG068_05800) - 1124263..1124844 (+) 582 WP_342718512.1 competence protein ComK -
  AAG068_RS05805 (AAG068_05805) - 1125153..1125746 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  AAG068_RS05810 (AAG068_05810) lepB 1125803..1126366 (+) 564 WP_342718517.1 signal peptidase I -
  AAG068_RS05815 (AAG068_05815) addB 1126483..1129998 (+) 3516 WP_342718518.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAG068_RS05820 (AAG068_05820) addA 1129995..1133720 (+) 3726 WP_342718519.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAG068_RS05825 (AAG068_05825) - 1133733..1134020 (+) 288 WP_342718520.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  AAG068_RS05830 (AAG068_05830) gerPF 1134100..1134315 (-) 216 WP_001141566.1 spore germination protein GerPF -
  AAG068_RS05835 (AAG068_05835) - 1134357..1134743 (-) 387 WP_342718521.1 spore germination protein GerPE -
  AAG068_RS05840 (AAG068_05840) gerPD 1134759..1134953 (-) 195 WP_342718522.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134251.35 Da        Isoelectric Point: 5.2121

>NTDB_id=989310 AAG068_RS05815 WP_342718518.1 1126483..1129998(+) (addB) [Bacillus paramycoides strain JYZ-SD5]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETLLYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQSLAHLEAHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVAGEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIIRGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSILVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=989310 AAG068_RS05815 WP_342718518.1 1126483..1129998(+) (addB) [Bacillus paramycoides strain JYZ-SD5]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACATTATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGGTTAGCGTGGAAGGTACTGCAAGAAGTCGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCAGAGTTTAAACGTTACA
ACGTGACGCCATCGAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTCGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CCTACAATTGCTAGTAGAAAAGCTTCCGCAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAAGAGCTAGAAATTGTAAGACAGCTTATGATTTGCGGCGCGAGAGTTACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCGCAATCATTAGCGCATT
TAGAAGCGCACTACGAAGCACGACCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGCACAGCCGCGAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGGCTTGTGGCAGGTGAAGACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGAACGCTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAGCGCCCTATGTCACATCATCCACTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGGGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCATGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGATTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAGGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACCGACGTTATGTCTACAGGACTTGAGGCACTTCAATTTGCCAACATTCCACCGTCATTAGA
TCAAGTGTTAATTGCAAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGTGTGAATGAAGGTG
TGATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGACGTTCTTAGTGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATATGTAGCAACGCCA
GAAGTAACGTTGTCATATGTAATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTATCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCATTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTATTTTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCGAAATCAAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTAGATAAGGCAGAAGATGAAAACGGT
ACGTTCCTACGTATTATTGATTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCTTCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATTGTTGAGATGAAAGGTGACGCATCTGAAGCGGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTGCTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCTACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGAAGCTTTAGTGCGCGTTCGAGTATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATACGTACACCATTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTGATTGATATTGCCCCT
TATAAAAAAGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492

Multiple sequence alignment