Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   AAG992_RS06960 Genome accession   NZ_CP152403
Coordinates   1489228..1489773 (-) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain P154     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1484228..1494773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG992_RS06935 (AAG992_06940) - 1484442..1484879 (-) 438 WP_005758199.1 isoprenylcysteine carboxylmethyltransferase family protein -
  AAG992_RS06940 (AAG992_06945) - 1485088..1485699 (-) 612 WP_005725137.1 YfgM family protein -
  AAG992_RS06945 (AAG992_06950) hisS 1485718..1486989 (-) 1272 WP_041423113.1 histidine--tRNA ligase -
  AAG992_RS06950 (AAG992_06955) ispG 1487019..1488122 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AAG992_RS06955 (AAG992_06960) - 1488149..1489105 (-) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  AAG992_RS06960 (AAG992_06965) pilF/pilF2 1489228..1489773 (-) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AAG992_RS06965 (AAG992_06970) - 1489812..1490996 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  AAG992_RS06970 (AAG992_06975) - 1491176..1492429 (-) 1254 WP_014391407.1 AI-2E family transporter -
  AAG992_RS06975 (AAG992_06980) glnB 1492430..1492768 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  AAG992_RS06980 (AAG992_06985) mog 1492813..1493391 (-) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  AAG992_RS06985 (AAG992_06990) - 1493417..1494346 (-) 930 WP_170460663.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=988889 AAG992_RS06960 WP_014391406.1 1489228..1489773(-) (pilF/pilF2) [Pasteurella multocida strain P154]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=988889 AAG992_RS06960 WP_014391406.1 1489228..1489773(-) (pilF/pilF2) [Pasteurella multocida strain P154]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47

Multiple sequence alignment