Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AAGT87_RS15600 Genome accession   NZ_CP152071
Coordinates   2359822..2360697 (-) Length   291 a.a.
NCBI ID   WP_000418747.1    Uniprot ID   H9L4S3
Organism   Vibrio cholerae strain Sudan_2019_18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2354822..2365697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT87_RS15580 (AAGT87_15585) parC 2355772..2358057 (+) 2286 WP_341264802.1 DNA topoisomerase IV subunit A -
  AAGT87_RS15585 (AAGT87_15590) yacG 2358106..2358303 (-) 198 WP_000162868.1 DNA gyrase inhibitor YacG -
  AAGT87_RS15590 (AAGT87_15595) zapD 2358445..2359185 (-) 741 WP_080876404.1 cell division protein ZapD -
  AAGT87_RS15595 (AAGT87_15600) coaE 2359217..2359825 (-) 609 WP_341260973.1 dephospho-CoA kinase -
  AAGT87_RS15600 (AAGT87_15605) pilD 2359822..2360697 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  AAGT87_RS15605 (AAGT87_15610) pilC 2360755..2361981 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  AAGT87_RS15610 (AAGT87_15615) pilB 2362024..2363714 (-) 1691 Protein_2041 type IV-A pilus assembly ATPase PilB -
  AAGT87_RS15615 (AAGT87_15620) pilA 2363714..2364175 (-) 462 WP_000649326.1 pilin Machinery gene
  AAGT87_RS15620 (AAGT87_15625) nadC 2364420..2365310 (-) 891 WP_353495249.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32433.69 Da        Isoelectric Point: 7.1618

>NTDB_id=987138 AAGT87_RS15600 WP_000418747.1 2359822..2360697(-) (pilD) [Vibrio cholerae strain Sudan_2019_18]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=987138 AAGT87_RS15600 WP_000418747.1 2359822..2360697(-) (pilD) [Vibrio cholerae strain Sudan_2019_18]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGTCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTTTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGTTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9L4S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

100

100

1
  pilD Vibrio campbellii strain DS40M4

75.347

98.969

0.746
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment