Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI16_RS11450 Genome accession   NZ_CP152044
Coordinates   2247482..2248552 (-) Length   356 a.a.
NCBI ID   WP_081106456.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0923     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2242482..2253552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI16_RS11400 (MHI16_11400) - 2242582..2243154 (-) 573 WP_058014687.1 signal peptidase I -
  MHI16_RS11405 (MHI16_11405) tapA 2243199..2243726 (-) 528 WP_058014686.1 amyloid fiber anchoring/assembly protein TapA -
  MHI16_RS11410 (MHI16_11410) - 2243993..2244304 (+) 312 WP_061410249.1 YqzG/YhdC family protein -
  MHI16_RS11415 (MHI16_11415) - 2244338..2244532 (-) 195 WP_044140973.1 YqzE family protein -
  MHI16_RS11420 (MHI16_11420) comGG 2244592..2244975 (-) 384 WP_058014684.1 competence type IV pilus minor pilin ComGG -
  MHI16_RS11425 (MHI16_11425) comGF 2244972..2245472 (-) 501 WP_061408553.1 competence type IV pilus minor pilin ComGF -
  MHI16_RS11430 (MHI16_11430) comGE 2245411..2245728 (-) 318 WP_058014682.1 competence type IV pilus minor pilin ComGE -
  MHI16_RS11435 (MHI16_11435) comGD 2245712..2246158 (-) 447 WP_061408555.1 competence type IV pilus minor pilin ComGD -
  MHI16_RS11440 (MHI16_11440) comGC 2246151..2246444 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI16_RS11445 (MHI16_11445) comGB 2246461..2247501 (-) 1041 WP_061408557.1 competence type IV pilus assembly protein ComGB -
  MHI16_RS11450 (MHI16_11450) comGA 2247482..2248552 (-) 1071 WP_081106456.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI16_RS11455 (MHI16_11455) - 2248812..2249402 (-) 591 WP_058014679.1 hypothetical protein -
  MHI16_RS11460 (MHI16_11460) - 2249803..2250675 (-) 873 WP_058014678.1 STAS domain-containing protein -
  MHI16_RS11470 (MHI16_11470) - 2250899..2251279 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI16_RS11475 (MHI16_11475) - 2251493..2251735 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI16_RS11480 (MHI16_11480) - 2251781..2252419 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  MHI16_RS11485 (MHI16_11485) - 2252577..2252750 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI16_RS11490 (MHI16_11490) - 2252775..2253089 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40352.75 Da        Isoelectric Point: 9.2484

>NTDB_id=986612 MHI16_RS11450 WP_081106456.1 2247482..2248552(-) (comGA) [Bacillus sp. FSL K6-0923]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986612 MHI16_RS11450 WP_081106456.1 2247482..2248552(-) (comGA) [Bacillus sp. FSL K6-0923]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGTATGAGGGCATCTGATGTGCATATTGTCCC
AAAAGAAAAGGAAGCGTCTGTATCTTTCCGAGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCCCATTTTAAATTTTTATCGTCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCGCTAGCT
GTGATGCTGAGAAGAGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAAAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCTACTCGATTATTGTCGTTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGCAAAACAACGACCCTCTATTCATTGATCCAGTTC
GCAAAAAAGAATTTTAACCGAAATATAATTACACTTGAAGATCCGGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTACAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGTC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTCGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment