Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHI46_RS05310 Genome accession   NZ_CP152042
Coordinates   1059657..1063361 (+) Length   1234 a.a.
NCBI ID   WP_342503149.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1054657..1068361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS05295 (MHI46_05295) - 1054964..1055374 (+) 411 WP_039177642.1 DoxX family protein -
  MHI46_RS05300 (MHI46_05300) - 1055571..1056017 (-) 447 WP_039177646.1 ferritin-like domain-containing protein -
  MHI46_RS05305 (MHI46_05305) addB 1056170..1059679 (+) 3510 WP_342503148.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHI46_RS05310 (MHI46_05310) addA 1059657..1063361 (+) 3705 WP_342503149.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHI46_RS05315 (MHI46_05315) sbcD 1063388..1064563 (+) 1176 WP_039177655.1 exonuclease subunit SbcD -
  MHI46_RS05320 (MHI46_05320) - 1064560..1067955 (+) 3396 WP_251181144.1 SMC family ATPase -
  MHI46_RS05325 (MHI46_05325) - 1067975..1068274 (+) 300 WP_039177660.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142352.41 Da        Isoelectric Point: 4.9068

>NTDB_id=986470 MHI46_RS05310 WP_342503149.1 1059657..1063361(+) (addA) [Bacillus sp. FSL K6-1000]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKKIYEFSRSHPSPEQWMRAFLSLYDVDVQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEYAADGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRQLVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHAVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKEQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIALPSDEPYDDSRIQQLLDSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRFTDGLEAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNIEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=986470 MHI46_RS05310 WP_342503149.1 1059657..1063361(+) (addA) [Bacillus sp. FSL K6-1000]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCGGCGGCAGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCGAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAGGTTCTGCGCACCTTTTATTATGAAATTGACCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTATTGGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCG
TTCTTTGAACTGGTTGATCGCTATACTTCTGACCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAAAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTTCTTTCTCTTTATGATGTAGATGTTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTACTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGTCCAGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAACTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAGAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGATGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ATATGCTGCAGACGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGT
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGACAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGTTCTATGCCATGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCTTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTGTTAAAAGTCATTGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCACTTGTCCATTTAGACGAAAATGAAATGGCACTGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAATGGCGTGATTTCTCGATGAATCATGCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATTTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAATCTCCGCGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTTCGCATGATGACAATTCACAGCAGTAAAGGG
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAGCTAGGTTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGGAAAGAGCTGTTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGATTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCATCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGGAGCAGCAAGACGAGGTGGTACAAGCTTTGATGGAGGGGCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
GCCACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTACGATGATTCACGTATACAGCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCGAAAAGCGGACTGGGTAAAACGAGAAGTGTCATTTAGTATGGTATTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATTTACAGACGGCCTTGAAGCAGCTGTGCCTATATTGAAAAAACGCTATGAA
ACTCAAATCGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATATAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.182

100

0.665

Multiple sequence alignment