Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHI46_RS05305 Genome accession   NZ_CP152042
Coordinates   1056170..1059679 (+) Length   1169 a.a.
NCBI ID   WP_342503148.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1051170..1064679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS05275 (MHI46_05275) lepB 1051688..1052200 (+) 513 WP_039177636.1 signal peptidase I -
  MHI46_RS05280 (MHI46_05280) - 1052239..1053384 (-) 1146 WP_058838811.1 AbrB family transcriptional regulator -
  MHI46_RS05285 (MHI46_05285) - 1053652..1054074 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  MHI46_RS05290 (MHI46_05290) - 1054067..1054906 (+) 840 WP_039177640.1 M56 family metallopeptidase -
  MHI46_RS05295 (MHI46_05295) - 1054964..1055374 (+) 411 WP_039177642.1 DoxX family protein -
  MHI46_RS05300 (MHI46_05300) - 1055571..1056017 (-) 447 WP_039177646.1 ferritin-like domain-containing protein -
  MHI46_RS05305 (MHI46_05305) addB 1056170..1059679 (+) 3510 WP_342503148.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHI46_RS05310 (MHI46_05310) addA 1059657..1063361 (+) 3705 WP_342503149.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHI46_RS05315 (MHI46_05315) sbcD 1063388..1064563 (+) 1176 WP_039177655.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134916.90 Da        Isoelectric Point: 5.3492

>NTDB_id=986469 MHI46_RS05305 WP_342503148.1 1056170..1059679(+) (addB) [Bacillus sp. FSL K6-1000]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLDPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAGE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISVVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGDEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLITSTHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGVLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDIEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=986469 MHI46_RS05305 WP_342503148.1 1056170..1059679(+) (addB) [Bacillus sp. FSL K6-1000]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTAGATCCGCTGGATCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATTAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTAATTGAAGAAGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCAGGGTTTGTCGAGCAAATCGAAAAGACGATGGCTGAGTTCAAAAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGGCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTCTATCTGAAGAAATAAAAGGAGCACATATTTATATAGATGGCTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTTCATGCTGAGAAAATAACGGCTGCGTTCACAGTGGATCGTTCC
TACCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACTGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGGCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTGTCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCAGT
TGTCGCACGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCAATGCAGTATCACCCGCTTATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAAACTGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCACTACAGACGAT
TCCAATCTTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATTGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCAGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGATGAGGAGCTC
TCATTTCCTTTATTCCAGCAAATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCAAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATTT
GTCTTATTCAGTGTCTGATGAAGAGGGGCGCTCTTTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATCAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCTTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTTTA
TAATTATTTGATAACGTCAACTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCAAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTTAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACAAAGG
ATGACTGTGTCAATTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTCTTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAACTCCAGAAAATCTTAATTCGAGTCTCTTCTATCTTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGACTTGAGTTAGGCTTTGGCGGCCAAGGACCTCTTCCTCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGAGTAGATAAAGCAGAAGGTTCAAAAGGGGTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAAAAAGGTCTTGATCTAGCAGAGGTATACTATGGGCTGGCTTTGCAAATGCTCACTTACTTAGA
TTTAACCATTACGTATTCGAAGGAATGGCTCGGTGTAGAAGCAACGCCAGCTGGCATTTTATATTTCCATATCCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATATAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGTTT
GAAAAAGGACGGATCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCACGTTCGGC
GTACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGTGAAGTTGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.805

99.316

0.654

Multiple sequence alignment