Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHB83_RS16175 Genome accession   NZ_CP152034
Coordinates   3184707..3185777 (-) Length   356 a.a.
NCBI ID   WP_342496475.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3149     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179707..3190777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB83_RS16125 (MHB83_16125) - 3179800..3180372 (-) 573 WP_342496474.1 signal peptidase I -
  MHB83_RS16130 (MHB83_16130) tapA 3180424..3180951 (-) 528 WP_137054980.1 amyloid fiber anchoring/assembly protein TapA -
  MHB83_RS16135 (MHB83_16135) - 3181251..3181529 (+) 279 WP_003217457.1 DUF3889 domain-containing protein -
  MHB83_RS16140 (MHB83_16140) - 3181563..3181757 (-) 195 WP_034620826.1 YqzE family protein -
  MHB83_RS16145 (MHB83_16145) comGG 3181817..3182200 (-) 384 WP_137054979.1 competence type IV pilus minor pilin ComGG -
  MHB83_RS16150 (MHB83_16150) comGF 3182197..3182697 (-) 501 WP_137054978.1 competence type IV pilus minor pilin ComGF -
  MHB83_RS16155 (MHB83_16155) comGE 3182636..3182953 (-) 318 WP_003217390.1 competence type IV pilus minor pilin ComGE -
  MHB83_RS16160 (MHB83_16160) comGD 3182937..3183383 (-) 447 WP_003217424.1 competence type IV pilus minor pilin ComGD -
  MHB83_RS16165 (MHB83_16165) comGC 3183376..3183669 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  MHB83_RS16170 (MHB83_16170) comGB 3183686..3184726 (-) 1041 WP_137054977.1 competence type IV pilus assembly protein ComGB -
  MHB83_RS16175 (MHB83_16175) comGA 3184707..3185777 (-) 1071 WP_342496475.1 competence type IV pilus ATPase ComGA Machinery gene
  MHB83_RS16180 (MHB83_16180) - 3185955..3186827 (-) 873 WP_003217438.1 STAS domain-containing protein -
  MHB83_RS16190 (MHB83_16190) - 3187051..3187431 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHB83_RS16195 (MHB83_16195) - 3187644..3187886 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHB83_RS16200 (MHB83_16200) - 3188131..3188769 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  MHB83_RS16205 (MHB83_16205) - 3188927..3189100 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHB83_RS16210 (MHB83_16210) - 3189325..3189639 (-) 315 WP_003217504.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40383.87 Da        Isoelectric Point: 9.5361

>NTDB_id=986026 MHB83_RS16175 WP_342496475.1 3184707..3185777(-) (comGA) [Bacillus sp. FSL K6-3149]
MYGIEYLGQELLEEACRMRASDVHIVPKEKKASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQMFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986026 MHB83_RS16175 WP_342496475.1 3184707..3185777(-) (comGA) [Bacillus sp. FSL K6-3149]
TTGTATGGGATTGAATACTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGAAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGATGTTTATTCGGATGTCTACCCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCATCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCGGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACATTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638

Multiple sequence alignment