Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHH61_RS14180 Genome accession   NZ_CP152033
Coordinates   2763015..2766719 (+) Length   1234 a.a.
NCBI ID   WP_125586545.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3154     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2758015..2771719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH61_RS14165 (MHH61_14150) - 2758323..2758733 (+) 411 WP_024425497.1 DoxX family protein -
  MHH61_RS14170 (MHH61_14155) - 2758929..2759375 (-) 447 WP_024425498.1 ferritin-like domain-containing protein -
  MHH61_RS14175 (MHH61_14160) addB 2759528..2763037 (+) 3510 WP_125586547.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHH61_RS14180 (MHH61_14165) addA 2763015..2766719 (+) 3705 WP_125586545.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHH61_RS14185 (MHH61_14170) sbcD 2766746..2767921 (+) 1176 WP_024426531.1 exonuclease subunit SbcD -
  MHH61_RS14190 (MHH61_14175) - 2767918..2771313 (+) 3396 WP_125586543.1 AAA family ATPase -
  MHH61_RS14195 (MHH61_14180) - 2771334..2771633 (+) 300 WP_024425503.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142298.47 Da        Isoelectric Point: 5.0587

>NTDB_id=985958 MHH61_RS14180 WP_125586545.1 2763015..2766719(+) (addA) [Bacillus sp. FSL K6-3154]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELVKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPDQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFKPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAESDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGEDWLIPDFERYQSKTYLDFIGPALIRHQAMSSILEETGNVVLSHPSVFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRRAH
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLSSDEPLDEIHIHQLLDSLQQRDLLTNEQVQSIDQEGISAFFSTSIGQ
KLLKADWVKREMSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAQAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=985958 MHH61_RS14180 WP_125586545.1 2763015..2766719(+) (addA) [Bacillus sp. FSL K6-3154]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGTCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCACTCATGAATCGTGCCAATATTTC
TACCCTGCACTCCTTTTGCTTGCAGGTTCTGCGCACCTTTTACTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAGGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGGCATGATTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGACCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTAGAACTGCTCGAACGAGCT
CTCGCTCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCGGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGAAGTCCCGCTCAGCATCTGAAAAGCTTAGCTGAAATGAAGCCAGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGATTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAATACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAAGATACAAACCTTGTACAGGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAACCCATGC
TCTTTTTAAACAAATATAAACAATTTAAACCAGATGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTCCTTGTTCATTTAG
ATCAACAAGAAGCAGAAAGTGACGAAGAGCGAGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTAGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAATTGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAACAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTATTAAAAGTCATCGATAATCCATATCAG
GATATCCCTCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGTACGAGTGA
TAAGAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGATTTTTCAGGTTGCTGCGCAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGTAAACAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCGGCCTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGTTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAAACGGAGAGGACTGGCTGAT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTTATCCGTCACCAAGCGATGTCAT
CTATTTTAGAAGAAACAGGTAATGTCGTTCTGTCGCATCCATCAGTATTCACAATCTCATTTACTCAAGCTTCTGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGCTTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCGTATCTAGCGGCCTCACAAGTCGGGACAA
AGCAGTCTGTTTCAGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTAGAAGAGCTCAT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTTCTTCTGACGAGCCCCTCGATGAAATCCATATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTTCAGTCAATTGATCAAGAAGGAATTTCCGCTTTTTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAATGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTAATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTGTATGCACAAGCGGTGGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.262

100

0.664

Multiple sequence alignment