Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHH61_RS14175 Genome accession   NZ_CP152033
Coordinates   2759528..2763037 (+) Length   1169 a.a.
NCBI ID   WP_125586547.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3154     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2754528..2768037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH61_RS14145 (MHH61_14130) lepB 2755048..2755560 (+) 513 WP_024425493.1 signal peptidase I -
  MHH61_RS14150 (MHH61_14135) - 2755599..2756744 (-) 1146 WP_024426536.1 AbrB family transcriptional regulator -
  MHH61_RS14155 (MHH61_14140) - 2757011..2757433 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  MHH61_RS14160 (MHH61_14145) - 2757426..2758265 (+) 840 WP_024426535.1 M56 family metallopeptidase -
  MHH61_RS14165 (MHH61_14150) - 2758323..2758733 (+) 411 WP_024425497.1 DoxX family protein -
  MHH61_RS14170 (MHH61_14155) - 2758929..2759375 (-) 447 WP_024425498.1 ferritin-like domain-containing protein -
  MHH61_RS14175 (MHH61_14160) addB 2759528..2763037 (+) 3510 WP_125586547.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHH61_RS14180 (MHH61_14165) addA 2763015..2766719 (+) 3705 WP_125586545.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHH61_RS14185 (MHH61_14170) sbcD 2766746..2767921 (+) 1176 WP_024426531.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134847.91 Da        Isoelectric Point: 5.3474

>NTDB_id=985957 MHH61_RS14175 WP_125586547.1 2759528..2763037(+) (addB) [Bacillus sp. FSL K6-3154]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTC
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLARECGAAISEHILEGNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGAAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKHLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEVGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=985957 MHH61_RS14175 WP_125586547.1 2759528..2763037(+) (addB) [Bacillus sp. FSL K6-3154]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGGAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTTGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCAGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCTGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTACACTCAGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCACAGCAGCTCCTCGTCATTGAACAGCTTCTGTTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCAAG
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGGGAAATAACCGTCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTATCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAGGGAGCAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCATTACAGACGAT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAGTTGCAAAAAAGGCTGAAAAAAGCACAAAAGGTCAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCAGAAAAACTTGAAAAGGCGAGGCTGGAGGCTGAGGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTAATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTTCAACAGATGATGGATACAGGACTGGCCTCTCTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGCATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACCGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTTGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGCTTTCGAGTCCATCCCATCATTTGTATCT
GTCTTATTCGGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCGA
ATCATCAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCTTATACCGTCTCGCAGCTGCAGCAATGGCTCAATCAATATCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTTTCAAAAAATATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
ACTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCGTTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAAGACCGGCAATTTTATAAACTGGAAGCACCTGATATCGG
CCAGCTGTTTCACTCAGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGACTGTGTCACTTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATTTTAAGTGAGCATGCAAAAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGTCAAGGACCTTTGCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGCTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCCCTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAGGAATGGCTCGGCGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAGTTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGTAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTAAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGACAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTCGTGATAGACCGGATTAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterProScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.064

99.316

0.656

Multiple sequence alignment