Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI60_RS15015 Genome accession   NZ_CP152032
Coordinates   2943832..2944902 (+) Length   356 a.a.
NCBI ID   WP_111292287.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3200     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2938832..2949902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI60_RS14975 (MHI60_14975) - 2938835..2940088 (+) 1254 WP_144484636.1 MFS transporter -
  MHI60_RS14980 (MHI60_14980) - 2940165..2940479 (+) 315 WP_048238883.1 MTH1187 family thiamine-binding protein -
  MHI60_RS14985 (MHI60_14985) - 2940703..2940876 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI60_RS14990 (MHI60_14990) - 2941034..2941672 (+) 639 WP_144484634.1 MBL fold metallo-hydrolase -
  MHI60_RS14995 (MHI60_14995) - 2941720..2941962 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI60_RS15000 (MHI60_15000) - 2942175..2942555 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI60_RS15010 (MHI60_15010) - 2942779..2943654 (+) 876 WP_144484632.1 STAS domain-containing protein -
  MHI60_RS15015 (MHI60_15015) comGA 2943832..2944902 (+) 1071 WP_111292287.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI60_RS15020 (MHI60_15020) comGB 2944883..2945923 (+) 1041 WP_144484630.1 competence type IV pilus assembly protein ComGB -
  MHI60_RS15025 (MHI60_15025) comGC 2945940..2946233 (+) 294 WP_144484628.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI60_RS15030 (MHI60_15030) comGD 2946226..2946672 (+) 447 WP_144484626.1 competence type IV pilus minor pilin ComGD -
  MHI60_RS15035 (MHI60_15035) comGE 2946656..2946970 (+) 315 WP_144484624.1 competence type IV pilus minor pilin ComGE -
  MHI60_RS15040 (MHI60_15040) comGF 2946882..2947409 (+) 528 WP_186437312.1 competence type IV pilus minor pilin ComGF -
  MHI60_RS15045 (MHI60_15045) comGG 2947406..2947789 (+) 384 WP_144484620.1 competence type IV pilus minor pilin ComGG -
  MHI60_RS15050 (MHI60_15050) - 2947849..2948043 (+) 195 WP_024718846.1 YqzE family protein -
  MHI60_RS15055 (MHI60_15055) - 2948077..2948355 (-) 279 WP_223251017.1 DUF3889 domain-containing protein -
  MHI60_RS15060 (MHI60_15060) tapA 2948655..2949182 (+) 528 WP_095408998.1 amyloid fiber anchoring/assembly protein TapA -
  MHI60_RS15065 (MHI60_15065) - 2949235..2949807 (+) 573 WP_144484618.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40484.98 Da        Isoelectric Point: 9.4799

>NTDB_id=985905 MHI60_RS15015 WP_111292287.1 2943832..2944902(+) (comGA) [Bacillus sp. FSL K6-3200]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPYRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=985905 MHI60_RS15015 WP_111292287.1 2943832..2944902(+) (comGA) [Bacillus sp. FSL K6-3200]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAACTAGATTATTATCATTTTTGA
AGCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTAATCCAGTTT
GCAAAAAAGAATTTTAATCGAAATATTATTACCCTTGAAGACCCTGTCGAAACGAGGAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTGG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTACAGACGAACAAATGTATTTGAATTGCTTTATGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646

Multiple sequence alignment