Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MHI60_RS14735 Genome accession   NZ_CP152032
Coordinates   2893351..2894121 (+) Length   256 a.a.
NCBI ID   WP_095409043.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-3200     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2888351..2899121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI60_RS14705 (MHI60_14705) - 2888682..2890769 (+) 2088 WP_186318661.1 HD family phosphohydrolase -
  MHI60_RS14710 (MHI60_14710) ybeY 2890770..2891249 (+) 480 WP_061408615.1 rRNA maturation RNase YbeY -
  MHI60_RS14715 (MHI60_14715) - 2891227..2891613 (+) 387 WP_024423020.1 diacylglycerol kinase family protein -
  MHI60_RS14720 (MHI60_14720) - 2891727..2892125 (+) 399 WP_008356263.1 cytidine deaminase -
  MHI60_RS14725 (MHI60_14725) era 2892118..2893023 (+) 906 WP_007501305.1 GTPase Era -
  MHI60_RS14730 (MHI60_14730) - 2893172..2893315 (+) 144 WP_003217288.1 YqzL family protein -
  MHI60_RS14735 (MHI60_14735) recO 2893351..2894121 (+) 771 WP_095409043.1 DNA repair protein RecO Machinery gene
  MHI60_RS14740 (MHI60_14740) glyQ 2894450..2895337 (+) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  MHI60_RS14745 (MHI60_14745) glyS 2895330..2897375 (+) 2046 WP_144484662.1 glycine--tRNA ligase subunit beta -
  MHI60_RS14750 (MHI60_14750) - 2897543..2898181 (+) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  MHI60_RS14755 (MHI60_14755) - 2898207..2899028 (+) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29288.93 Da        Isoelectric Point: 8.1204

>NTDB_id=985904 MHI60_RS14735 WP_095409043.1 2893351..2894121(+) (recO) [Bacillus sp. FSL K6-3200]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=985904 MHI60_RS14735 WP_095409043.1 2893351..2894121(+) (recO) [Bacillus sp. FSL K6-3200]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCAAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCTATAAGCCAGCCGTTTT
TATACGGTACATTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGACTTATTTCTGACCGCTTATGCTGCATATATGACGGAATTACTTGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAATGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTGGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGATTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCTGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTCGATGTGAAGCCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment