Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MKX52_RS05080 Genome accession   NZ_CP152014
Coordinates   1028849..1032553 (+) Length   1234 a.a.
NCBI ID   WP_342491599.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0422     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1023849..1037553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX52_RS05065 (MKX52_05065) - 1024144..1024554 (+) 411 WP_025093362.1 DoxX family protein -
  MKX52_RS05070 (MKX52_05070) - 1024751..1025197 (-) 447 WP_342491597.1 ferritin-like domain-containing protein -
  MKX52_RS05075 (MKX52_05075) addB 1025362..1028871 (+) 3510 WP_342491598.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKX52_RS05080 (MKX52_05080) addA 1028849..1032553 (+) 3705 WP_342491599.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKX52_RS05085 (MKX52_05085) sbcD 1032580..1033755 (+) 1176 WP_012009496.1 exonuclease subunit SbcD -
  MKX52_RS05090 (MKX52_05090) - 1033752..1037147 (+) 3396 WP_342491600.1 SMC family ATPase -
  MKX52_RS05095 (MKX52_05095) - 1037167..1037466 (+) 300 WP_012009498.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142292.27 Da        Isoelectric Point: 5.0923

>NTDB_id=984952 MKX52_RS05080 WP_342491599.1 1028849..1032553(+) (addA) [Bacillus sp. FSL R5-0422]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRSEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSSEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLNQQETETDEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDLSLEKKEQDEIVQALMVGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMREIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQVQSINQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGIDAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNVEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=984952 MKX52_RS05080 WP_342491599.1 1028849..1032553(+) (addA) [Bacillus sp. FSL R5-0422]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
AGTTGCGGCGGCGGCAGGTTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAGATGACCCGGTCTGAACATC
CAGTTGATGTCGATCGTCTGCTCGTTGTGACTTTCACAAACGCATCTGCAGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTACAGGTTTTACGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTAGTTGATCGTTACACATCTGACCGTCATGACTTAGATTTGCAATGGCTCGTGAAGAGAATCTATGAGTT
TTCAAGATCGCATCCATCTCCGGAGCAATGGATGCGGGCGTTCCTTTCACTATATGATGTAGATGCGCAAACAAAAGTGG
AAGAATTACCATTTTACCCCTATATCAAAGAAGATCTTTCGCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCCGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGACGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGATCCTGCTTTATTAGAGAAAGCCACTGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTCGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCTATTGTCGACTTTTCCGATTTAGAGCACT
ATTGCTTACGTATTTTAGCTGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACAATTTTAAAACTTGTATCTAAAGGAGA
ACATTCTTCAGAGGGTAATCTGTTTATGGTTGGTGATGTCAAGCAGTCTATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAA
ATCAGCAGGAAACGGAAACTGACGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGGAACTTGCAGTATCGAGACAT
TGTCATTTTACTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATCCTTTCTTTGTTAAAAGTCATTGATAATCCATATCAG
GATATTCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAGTTGGCGCTGATTCGGACGAGTGA
TAAAAAGGGCACGTATTATGATGCTGTAAAGGCCTTTATGAGTGTGACTCATTCTGATCATCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAATGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGCGATACACAGTATCTTGATTATGTTGGGGGAATGCCTGGAGGTAAGCAGCGGCAAGCGAATCTTCGTGCCCTTTATGA
CCGGGCCAAGCAGTACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGACGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATTCATAGTAGTAAAGGA
TTAGAATTCCCAGTCGTCTTTACCGCAGGACTTGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAGCTAGGCTTTGGAAGTAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAGTTGAGGGTTCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTCGTCGGCTCTGTCAAAAATCAGGTAAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCAGATTTTGAACGGTATCAATCGAAAACATACTTAGATTTCATTGGACCTGCCCTCATACGTCATCAAGCGATGTCAT
CTGTTCTAGAAGAAACAGGAGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATCTGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGTTGGTCTACCTGTTGAAGG
ATATGGCGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCGTGGAAATATCCGTATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAGAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCCATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCCTATGATGAGTCACGTATAGGTCACTTGCTCGAATCTTTACAACAGC
GAGACTTGCTAACAGATGAGCAGGTTCAGTCAATTAATCAAGAGGGAATCGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCATTTAGTATGGTCTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTTTAATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTTCAAGGCAGATATACAGGTGGAATTGACGCTGCTGTACCAATCTTGAAAAAGCGTTATGAA
ACCCAAATCGCGTTATATGCAAAAGCGGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGA
TGGAAATGTAGAGATATCTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.748

100

0.669

Multiple sequence alignment