Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MKX52_RS05075 Genome accession   NZ_CP152014
Coordinates   1025362..1028871 (+) Length   1169 a.a.
NCBI ID   WP_342491598.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0422     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1020362..1033871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX52_RS05045 (MKX52_05045) lepB 1020873..1021385 (+) 513 WP_041815362.1 signal peptidase I -
  MKX52_RS05050 (MKX52_05050) - 1021421..1022566 (-) 1146 WP_342491871.1 AbrB family transcriptional regulator -
  MKX52_RS05055 (MKX52_05055) - 1022833..1023255 (+) 423 WP_041815366.1 BlaI/MecI/CopY family transcriptional regulator -
  MKX52_RS05060 (MKX52_05060) - 1023248..1024087 (+) 840 WP_342491596.1 M56 family metallopeptidase -
  MKX52_RS05065 (MKX52_05065) - 1024144..1024554 (+) 411 WP_025093362.1 DoxX family protein -
  MKX52_RS05070 (MKX52_05070) - 1024751..1025197 (-) 447 WP_342491597.1 ferritin-like domain-containing protein -
  MKX52_RS05075 (MKX52_05075) addB 1025362..1028871 (+) 3510 WP_342491598.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKX52_RS05080 (MKX52_05080) addA 1028849..1032553 (+) 3705 WP_342491599.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKX52_RS05085 (MKX52_05085) sbcD 1032580..1033755 (+) 1176 WP_012009496.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134829.83 Da        Isoelectric Point: 5.1979

>NTDB_id=984951 MKX52_RS05075 WP_342491598.1 1025362..1028871(+) (addB) [Bacillus sp. FSL R5-0422]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEEIEKISVESMLSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLNLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAARVTAAFTVDQS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISETIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSASK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKRLKKTQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSPLIKYCQELMPNHQQALYVLDPELLEDDEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGIEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=984951 MKX52_RS05075 WP_342491598.1 1025362..1028871(+) (addB) [Bacillus sp. FSL R5-0422]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTAGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCTGAGGAAATTGAGAAAATTTCAGTGGAGAGCATGCTTTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGACCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GAATCTACTTGCCCAGCAAATTCCTTCAGCAGAAGAAATAAAAGGAGCACATATTTATATCGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATCGAACAGCTCTTGCTGCATGCAGCAAGAGTAACGGCGGCTTTTACAGTAGATCAATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAACTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAACCATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTGCAACCATACCAGGAAAATACCCCTCATCTCACAGTGTCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAAGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTTAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATCGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTAAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCACGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTACGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGACAAACGGATCAAGAAATTGAAATGGAACAAATGCTGAATGACGTCAAAGAATGG
ATCGCGCCTCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAGACGCAAAAGGTAAGAGATATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTAGAAGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTCATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTTCAACAGATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTAATTCCGCCATCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCTTCTGATGAGAGTGTATTGTCTGAAGATGACCGAGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTATATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGCCACTTATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACTGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCTTATACCGTCTCACAGCTCCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAATATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGGCAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCGCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGACCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTAGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTCACCATTACGTATTCGAAGGAGTGGCTAGGCATAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGACC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGCTTA
TTACTTGAAGATGTGGAAGCAGTCAAGCTAATGGATCAAACGCTTGAGTCAGGTAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCGGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGTGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGCCAATTCGATGAATCGATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.805

99.316

0.654

Multiple sequence alignment