Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYE28_RS13180 Genome accession   NZ_CP152013
Coordinates   2664339..2665409 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0443     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659339..2670409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE28_RS13130 (NYE28_13130) - 2659399..2659983 (-) 585 WP_015240205.1 signal peptidase I -
  NYE28_RS13135 (NYE28_13135) tapA 2659955..2660626 (-) 672 WP_124934997.1 amyloid fiber anchoring/assembly protein TapA -
  NYE28_RS13140 (NYE28_13140) - 2660885..2661214 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NYE28_RS13145 (NYE28_13145) - 2661254..2661433 (-) 180 WP_003153093.1 YqzE family protein -
  NYE28_RS13150 (NYE28_13150) comGG 2661490..2661867 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYE28_RS13155 (NYE28_13155) comGF 2661868..2662368 (-) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  NYE28_RS13160 (NYE28_13160) comGE 2662277..2662591 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  NYE28_RS13165 (NYE28_13165) comGD 2662575..2663012 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYE28_RS13170 (NYE28_13170) comGC 2663002..2663310 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE28_RS13175 (NYE28_13175) comGB 2663315..2664352 (-) 1038 WP_337681036.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE28_RS13180 (NYE28_13180) comGA 2664339..2665409 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE28_RS13185 (NYE28_13185) - 2665603..2666553 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NYE28_RS13190 (NYE28_13190) - 2666699..2668000 (+) 1302 WP_021494315.1 hemolysin family protein -
  NYE28_RS13195 (NYE28_13195) - 2668038..2668868 (-) 831 WP_007408317.1 STAS domain-containing protein -
  NYE28_RS13205 (NYE28_13205) - 2669074..2669454 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYE28_RS13210 (NYE28_13210) - 2669692..2669937 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=984909 NYE28_RS13180 WP_003153083.1 2664339..2665409(-) (comGA) [Bacillus sp. FSL R5-0443]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=984909 NYE28_RS13180 WP_003153083.1 2664339..2665409(-) (comGA) [Bacillus sp. FSL R5-0443]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment