Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MKZ22_RS12665 Genome accession   NZ_CP152009
Coordinates   2439649..2440719 (-) Length   356 a.a.
NCBI ID   WP_044140966.1    Uniprot ID   A0AB34QT08
Organism   Bacillus sp. FSL R7-0675     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2434649..2445719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ22_RS12615 (MKZ22_12615) - 2434742..2435314 (-) 573 WP_044140976.1 signal peptidase I -
  MKZ22_RS12620 (MKZ22_12620) tapA 2435366..2435893 (-) 528 WP_044140975.1 amyloid fiber anchoring/assembly protein TapA -
  MKZ22_RS12625 (MKZ22_12625) - 2436193..2436471 (+) 279 WP_065098439.1 DUF3889 domain-containing protein -
  MKZ22_RS12630 (MKZ22_12630) - 2436505..2436699 (-) 195 WP_044140973.1 YqzE family protein -
  MKZ22_RS12635 (MKZ22_12635) comGG 2436759..2437142 (-) 384 WP_044140972.1 competence type IV pilus minor pilin ComGG -
  MKZ22_RS12640 (MKZ22_12640) comGF 2437139..2437639 (-) 501 WP_044140971.1 competence type IV pilus minor pilin ComGF -
  MKZ22_RS12645 (MKZ22_12645) comGE 2437578..2437895 (-) 318 WP_044140968.1 competence type IV pilus minor pilin ComGE -
  MKZ22_RS12650 (MKZ22_12650) comGD 2437879..2438325 (-) 447 WP_106065588.1 competence type IV pilus minor pilin ComGD -
  MKZ22_RS12655 (MKZ22_12655) comGC 2438318..2438611 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  MKZ22_RS12660 (MKZ22_12660) comGB 2438628..2439707 (-) 1080 WP_231129518.1 competence type IV pilus assembly protein ComGB -
  MKZ22_RS12665 (MKZ22_12665) comGA 2439649..2440719 (-) 1071 WP_044140966.1 competence type IV pilus ATPase ComGA Machinery gene
  MKZ22_RS12670 (MKZ22_12670) - 2440898..2441770 (-) 873 WP_065098399.1 STAS domain-containing protein -
  MKZ22_RS12680 (MKZ22_12680) - 2441994..2442374 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKZ22_RS12685 (MKZ22_12685) - 2442588..2442830 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MKZ22_RS12690 (MKZ22_12690) - 2442876..2443514 (-) 639 WP_065098400.1 MBL fold metallo-hydrolase -
  MKZ22_RS12695 (MKZ22_12695) - 2443672..2443845 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MKZ22_RS12700 (MKZ22_12700) - 2444068..2444382 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  MKZ22_RS12705 (MKZ22_12705) - 2444461..2445714 (-) 1254 WP_065098401.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40283.64 Da        Isoelectric Point: 9.0692

>NTDB_id=984628 MKZ22_RS12665 WP_044140966.1 2439649..2440719(-) (comGA) [Bacillus sp. FSL R7-0675]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=984628 MKZ22_RS12665 WP_044140966.1 2439649..2440719(-) (comGA) [Bacillus sp. FSL R7-0675]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATTGGAGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCCTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCCGAACGGCAATTAGAGCAGCACTAACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCATTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTCGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment