Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MKZ22_RS12655 Genome accession   NZ_CP152009
Coordinates   2438318..2438611 (-) Length   97 a.a.
NCBI ID   WP_003217322.1    Uniprot ID   -
Organism   Bacillus sp. FSL R7-0675     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2433318..2443611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ22_RS12605 (MKZ22_12605) sinR 2433480..2433815 (+) 336 WP_012010555.1 transcriptional regulator SinR Regulator
  MKZ22_RS12610 (MKZ22_12610) - 2433883..2434683 (-) 801 WP_044140977.1 TasA family protein -
  MKZ22_RS12615 (MKZ22_12615) - 2434742..2435314 (-) 573 WP_044140976.1 signal peptidase I -
  MKZ22_RS12620 (MKZ22_12620) tapA 2435366..2435893 (-) 528 WP_044140975.1 amyloid fiber anchoring/assembly protein TapA -
  MKZ22_RS12625 (MKZ22_12625) - 2436193..2436471 (+) 279 WP_065098439.1 DUF3889 domain-containing protein -
  MKZ22_RS12630 (MKZ22_12630) - 2436505..2436699 (-) 195 WP_044140973.1 YqzE family protein -
  MKZ22_RS12635 (MKZ22_12635) comGG 2436759..2437142 (-) 384 WP_044140972.1 competence type IV pilus minor pilin ComGG -
  MKZ22_RS12640 (MKZ22_12640) comGF 2437139..2437639 (-) 501 WP_044140971.1 competence type IV pilus minor pilin ComGF -
  MKZ22_RS12645 (MKZ22_12645) comGE 2437578..2437895 (-) 318 WP_044140968.1 competence type IV pilus minor pilin ComGE -
  MKZ22_RS12650 (MKZ22_12650) comGD 2437879..2438325 (-) 447 WP_106065588.1 competence type IV pilus minor pilin ComGD -
  MKZ22_RS12655 (MKZ22_12655) comGC 2438318..2438611 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  MKZ22_RS12660 (MKZ22_12660) comGB 2438628..2439707 (-) 1080 WP_231129518.1 competence type IV pilus assembly protein ComGB -
  MKZ22_RS12665 (MKZ22_12665) comGA 2439649..2440719 (-) 1071 WP_044140966.1 competence type IV pilus ATPase ComGA Machinery gene
  MKZ22_RS12670 (MKZ22_12670) - 2440898..2441770 (-) 873 WP_065098399.1 STAS domain-containing protein -
  MKZ22_RS12680 (MKZ22_12680) - 2441994..2442374 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKZ22_RS12685 (MKZ22_12685) - 2442588..2442830 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MKZ22_RS12690 (MKZ22_12690) - 2442876..2443514 (-) 639 WP_065098400.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10739.69 Da        Isoelectric Point: 7.3837

>NTDB_id=984627 MKZ22_RS12655 WP_003217322.1 2438318..2438611(-) (comGC) [Bacillus sp. FSL R7-0675]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQTIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=984627 MKZ22_RS12655 WP_003217322.1 2438318..2438611(-) (comGC) [Bacillus sp. FSL R7-0675]
ATGAATGATAAAGGATTTACTCTGTTAGAAATGTTAATTGTCATGCTTGTGATTTCGATTCTTTTACTTATTACCATTCC
GAATATTACTCGTCACCACCAAACGATTCAAGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTGAGCACTCAGGTGATGG
CATATGAATTAGAGCACGATGGCAAGACACCTTCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACG
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCATGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment