Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYE21_RS13115 Genome accession   NZ_CP151999
Coordinates   2536303..2537367 (-) Length   354 a.a.
NCBI ID   WP_073425737.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-8391     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531303..2542367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYE21_RS13070 (NYE21_13070) tapA 2531797..2532525 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  NYE21_RS13075 (NYE21_13075) - 2532835..2533122 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  NYE21_RS13080 (NYE21_13080) - 2533152..2533334 (-) 183 WP_003183456.1 YqzE family protein -
  NYE21_RS13085 (NYE21_13085) comGG 2533423..2533788 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  NYE21_RS13090 (NYE21_13090) comGF 2533801..2534289 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  NYE21_RS13095 (NYE21_13095) comGE 2534198..2534545 (-) 348 WP_026699160.1 competence type IV pilus minor pilin ComGE -
  NYE21_RS13100 (NYE21_13100) comGD 2534529..2534966 (-) 438 WP_026699159.1 competence type IV pilus minor pilin ComGD -
  NYE21_RS13105 (NYE21_13105) comGC 2534972..2535265 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  NYE21_RS13110 (NYE21_13110) comGB 2535279..2536313 (-) 1035 WP_026699158.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYE21_RS13115 (NYE21_13115) comGA 2536303..2537367 (-) 1065 WP_073425737.1 competence type IV pilus ATPase ComGA Machinery gene
  NYE21_RS13120 (NYE21_13120) - 2537524..2538363 (-) 840 WP_003183480.1 STAS domain-containing protein -
  NYE21_RS13130 (NYE21_13130) - 2538605..2538982 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYE21_RS13135 (NYE21_13135) - 2539191..2539436 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  NYE21_RS13140 (NYE21_13140) - 2539474..2540112 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -
  NYE21_RS13145 (NYE21_13145) - 2540268..2540441 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  NYE21_RS13150 (NYE21_13150) - 2540502..2540816 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  NYE21_RS13155 (NYE21_13155) - 2540832..2541941 (-) 1110 WP_003183488.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39758.32 Da        Isoelectric Point: 9.1029

>NTDB_id=984234 NYE21_RS13115 WP_073425737.1 2536303..2537367(-) (comGA) [Bacillus sp. FSL K6-8391]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDGLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINREPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIDY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLELGVDMTEIEQTLVAVSAQRLVDIVCQFCGDSCTLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GGIKTDTLKMLIRKGIALGYLPSKTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=984234 NYE21_RS13115 WP_073425737.1 2536303..2537367(-) (comGA) [Bacillus sp. FSL K6-8391]
TTGTACACGATCGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTACGCTTCAGGATTGATGACGAACTGTTTCAAAAAGACGGATTAACGAGAAATGAGTGCT
CTAGACTCATTTCTCACTTTAAATTTCTTTCTTCGATGGACATTGGCGAGAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTGAGCCCGTTCATTTAAGGATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTCCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTTTTCACCGGCCCGACAGGCTCGGGAAAAACGACAACACTCTATTCTTTAATTGACTAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCGGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACGGGACACCTTGTTCTATCGAGCATGCAT
GCGAAAAATGCTAAAGGTGCGATATACAGGCTGCTCGAGCTCGGCGTTGATATGACAGAAATTGAACAAACATTGGTCGC
AGTCAGCGCCCAGCGTCTTGTCGATATCGTCTGTCAGTTTTGCGGCGACAGCTGCACTTTGTATTGCAGATTGTCGAGAC
CAGTTAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCTTAATCTCTGCATCGAAGAAGCAAAAGGCAGATGC
GGTGGCATCAAAACAGACACCTTAAAAATGCTGATCCGCAAGGGAATAGCACTCGGATATCTGCCGTCGAAAACTTATGA
ACGCTGGATAGGGCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.667

Multiple sequence alignment