Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSQ70_RS11305 Genome accession   NZ_CP151992
Coordinates   2217753..2218823 (-) Length   356 a.a.
NCBI ID   WP_327871870.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0397     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2212753..2223823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ70_RS11255 (NSQ70_11255) - 2212848..2213420 (-) 573 WP_017357979.1 signal peptidase I -
  NSQ70_RS11260 (NSQ70_11260) tapA 2213470..2213997 (-) 528 WP_326111031.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ70_RS11265 (NSQ70_11265) - 2214250..2214576 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  NSQ70_RS11270 (NSQ70_11270) - 2214611..2214805 (-) 195 WP_342502160.1 YqzE family protein -
  NSQ70_RS11275 (NSQ70_11275) comGG 2214866..2215249 (-) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  NSQ70_RS11280 (NSQ70_11280) comGF 2215246..2215743 (-) 498 WP_039166031.1 competence type IV pilus minor pilin ComGF -
  NSQ70_RS11285 (NSQ70_11285) comGE 2215685..2215999 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  NSQ70_RS11290 (NSQ70_11290) comGD 2215983..2216429 (-) 447 WP_039166035.1 competence type IV pilus minor pilin ComGD -
  NSQ70_RS11295 (NSQ70_11295) comGC 2216422..2216715 (-) 294 WP_024718841.1 competence type IV pilus major pilin ComGC Machinery gene
  NSQ70_RS11300 (NSQ70_11300) comGB 2216732..2217772 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  NSQ70_RS11305 (NSQ70_11305) comGA 2217753..2218823 (-) 1071 WP_327871870.1 competence type IV pilus ATPase ComGA Machinery gene
  NSQ70_RS11310 (NSQ70_11310) - 2219282..2220154 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NSQ70_RS11320 (NSQ70_11320) - 2220380..2220760 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSQ70_RS11325 (NSQ70_11325) - 2220973..2221215 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NSQ70_RS11330 (NSQ70_11330) - 2221264..2221902 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  NSQ70_RS11335 (NSQ70_11335) - 2222063..2222236 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NSQ70_RS11340 (NSQ70_11340) - 2222261..2222575 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40352.65 Da        Isoelectric Point: 8.8590

>NTDB_id=983929 NSQ70_RS11305 WP_327871870.1 2217753..2218823(-) (comGA) [Bacillus sp. FSL R5-0397]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECINEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983929 NSQ70_RS11305 WP_327871870.1 2217753..2218823(-) (comGA) [Bacillus sp. FSL R5-0397]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTCAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAACGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment