Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST15_RS11425 Genome accession   NZ_CP151991
Coordinates   2226144..2227214 (-) Length   356 a.a.
NCBI ID   WP_224558453.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0820     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2221144..2232214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST15_RS11375 (NST15_11375) - 2221246..2221818 (-) 573 WP_017357979.1 signal peptidase I -
  NST15_RS11380 (NST15_11380) tapA 2221868..2222395 (-) 528 WP_050827403.1 amyloid fiber anchoring/assembly protein TapA -
  NST15_RS11385 (NST15_11385) - 2222648..2222974 (+) 327 WP_048001387.1 DUF3889 domain-containing protein -
  NST15_RS11390 (NST15_11390) - 2223008..2223196 (-) 189 WP_017367591.1 YqzE family protein -
  NST15_RS11395 (NST15_11395) comGG 2223257..2223640 (-) 384 WP_058214152.1 competence type IV pilus minor pilin ComGG -
  NST15_RS11400 (NST15_11400) comGF 2223637..2224134 (-) 498 WP_342498540.1 competence type IV pilus minor pilin ComGF -
  NST15_RS11405 (NST15_11405) comGE 2224076..2224390 (-) 315 WP_058214150.1 competence type IV pilus minor pilin ComGE -
  NST15_RS11410 (NST15_11410) comGD 2224374..2224820 (-) 447 WP_057079312.1 competence type IV pilus minor pilin ComGD -
  NST15_RS11415 (NST15_11415) comGC 2224813..2225106 (-) 294 WP_057079311.1 competence type IV pilus major pilin ComGC Machinery gene
  NST15_RS11420 (NST15_11420) comGB 2225123..2226163 (-) 1041 WP_342498541.1 competence type IV pilus assembly protein ComGB -
  NST15_RS11425 (NST15_11425) comGA 2226144..2227214 (-) 1071 WP_224558453.1 competence type IV pilus ATPase ComGA Machinery gene
  NST15_RS11430 (NST15_11430) - 2227671..2228543 (-) 873 WP_046527023.1 STAS domain-containing protein -
  NST15_RS11440 (NST15_11440) - 2228769..2229149 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST15_RS11445 (NST15_11445) - 2229362..2229604 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NST15_RS11450 (NST15_11450) - 2229653..2230291 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  NST15_RS11455 (NST15_11455) - 2230452..2230625 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NST15_RS11460 (NST15_11460) - 2230650..2230964 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40325.67 Da        Isoelectric Point: 9.0449

>NTDB_id=983867 NST15_RS11425 WP_224558453.1 2226144..2227214(-) (comGA) [Bacillus sp. FSL R5-0820]
MYGIEYLGQELLEDACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983867 NST15_RS11425 WP_224558453.1 2226144..2227214(-) (comGA) [Bacillus sp. FSL R5-0820]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGATGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATCATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTCCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646

Multiple sequence alignment