Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NSQ11_RS11540 Genome accession   NZ_CP151987
Coordinates   2244260..2244553 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL W7-1582     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2239260..2249553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ11_RS11490 (NSQ11_11490) - 2239419..2239745 (+) 327 Protein_2221 helix-turn-helix domain-containing protein -
  NSQ11_RS11495 (NSQ11_11495) - 2239821..2240621 (-) 801 WP_035390805.1 TasA family protein -
  NSQ11_RS11500 (NSQ11_11500) - 2240680..2241252 (-) 573 WP_041091749.1 signal peptidase I -
  NSQ11_RS11505 (NSQ11_11505) tapA 2241302..2241829 (-) 528 WP_046343966.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ11_RS11510 (NSQ11_11510) - 2242082..2242408 (+) 327 WP_048001387.1 DUF3889 domain-containing protein -
  NSQ11_RS11515 (NSQ11_11515) - 2242443..2242643 (-) 201 WP_060781188.1 YqzE family protein -
  NSQ11_RS11520 (NSQ11_11520) comGG 2242704..2243087 (-) 384 WP_057079315.1 competence type IV pilus minor pilin ComGG -
  NSQ11_RS11525 (NSQ11_11525) comGF 2243084..2243581 (-) 498 WP_342501107.1 competence type IV pilus minor pilin ComGF -
  NSQ11_RS11530 (NSQ11_11530) comGE 2243523..2243837 (-) 315 WP_057079313.1 competence type IV pilus minor pilin ComGE -
  NSQ11_RS11535 (NSQ11_11535) comGD 2243821..2244267 (-) 447 WP_342501108.1 competence type IV pilus minor pilin ComGD -
  NSQ11_RS11540 (NSQ11_11540) comGC 2244260..2244553 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NSQ11_RS11545 (NSQ11_11545) comGB 2244570..2245610 (-) 1041 WP_342501109.1 competence type IV pilus assembly protein ComGB -
  NSQ11_RS11550 (NSQ11_11550) comGA 2245591..2246661 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  NSQ11_RS11555 (NSQ11_11555) - 2247119..2247991 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NSQ11_RS11565 (NSQ11_11565) - 2248217..2248597 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSQ11_RS11570 (NSQ11_11570) - 2248810..2249052 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=983620 NSQ11_RS11540 WP_008342186.1 2244260..2244553(-) (comGC) [Bacillus sp. FSL W7-1582]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983620 NSQ11_RS11540 WP_008342186.1 2244260..2244553(-) (comGC) [Bacillus sp. FSL W7-1582]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATTACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGATGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACT
TGTCCGAATGGAAAGGCAATTTTCATTCAAAATGGGGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment