Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSQ25_RS11380 Genome accession   NZ_CP151982
Coordinates   2222075..2223145 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0512     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217075..2228145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ25_RS11330 (NSQ25_11330) - 2217168..2217740 (-) 573 WP_034280218.1 signal peptidase I -
  NSQ25_RS11335 (NSQ25_11335) tapA 2217793..2218320 (-) 528 WP_144496218.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ25_RS11340 (NSQ25_11340) - 2218621..2218899 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  NSQ25_RS11345 (NSQ25_11345) - 2218934..2219128 (-) 195 WP_024422974.1 YqzE family protein -
  NSQ25_RS11350 (NSQ25_11350) comGG 2219188..2219571 (-) 384 WP_326136240.1 competence type IV pilus minor pilin ComGG -
  NSQ25_RS11355 (NSQ25_11355) comGF 2219568..2220095 (-) 528 WP_260983449.1 competence type IV pilus minor pilin ComGF -
  NSQ25_RS11360 (NSQ25_11360) comGE 2220007..2220321 (-) 315 WP_034621606.1 competence type IV pilus minor pilin ComGE -
  NSQ25_RS11365 (NSQ25_11365) comGD 2220305..2220751 (-) 447 WP_070326501.1 competence type IV pilus minor pilin ComGD -
  NSQ25_RS11370 (NSQ25_11370) comGC 2220744..2221037 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  NSQ25_RS11375 (NSQ25_11375) comGB 2221054..2222094 (-) 1041 WP_034280202.1 competence type IV pilus assembly protein ComGB -
  NSQ25_RS11380 (NSQ25_11380) comGA 2222075..2223145 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  NSQ25_RS11385 (NSQ25_11385) - 2223323..2224198 (-) 876 WP_024422979.1 STAS domain-containing protein -
  NSQ25_RS11395 (NSQ25_11395) - 2224422..2224802 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSQ25_RS11400 (NSQ25_11400) - 2225015..2225257 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NSQ25_RS11405 (NSQ25_11405) - 2225302..2225940 (-) 639 WP_034280195.1 MBL fold metallo-hydrolase -
  NSQ25_RS11410 (NSQ25_11410) - 2226098..2226271 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NSQ25_RS11415 (NSQ25_11415) - 2226295..2226609 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  NSQ25_RS11420 (NSQ25_11420) - 2226686..2227939 (-) 1254 WP_065214634.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=983315 NSQ25_RS11380 WP_041117979.1 2222075..2223145(-) (comGA) [Bacillus sp. FSL R5-0512]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983315 NSQ25_RS11380 WP_041117979.1 2222075..2223145(-) (comGA) [Bacillus sp. FSL R5-0512]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCGCTAGCT
GTAATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTTGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAGCTGCTGTTCGGAAAAGAGCTTGGTGAATGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment