Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NSQ25_RS05195 Genome accession   NZ_CP151982
Coordinates   1036357..1040061 (+) Length   1234 a.a.
NCBI ID   WP_326136607.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0512     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1031357..1045061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ25_RS05180 (NSQ25_05180) - 1031664..1032074 (+) 411 WP_034282155.1 DoxX family protein -
  NSQ25_RS05185 (NSQ25_05185) - 1032271..1032717 (-) 447 WP_061109640.1 ferritin-like domain-containing protein -
  NSQ25_RS05190 (NSQ25_05190) addB 1032870..1036379 (+) 3510 WP_214755275.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSQ25_RS05195 (NSQ25_05195) addA 1036357..1040061 (+) 3705 WP_326136607.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSQ25_RS05200 (NSQ25_05200) sbcD 1040088..1041263 (+) 1176 WP_070325681.1 exonuclease subunit SbcD -
  NSQ25_RS05205 (NSQ25_05205) - 1041260..1044655 (+) 3396 WP_326136605.1 SMC family ATPase -
  NSQ25_RS05210 (NSQ25_05210) - 1044675..1044974 (+) 300 WP_326136604.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142421.52 Da        Isoelectric Point: 4.9714

>NTDB_id=983293 NSQ25_RS05195 WP_326136607.1 1036357..1040061(+) (addA) [Bacillus sp. FSL R5-0512]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQINELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVIETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILRLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIDYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKKQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKDR
ATMYDRPAFMKKKTLTAAEQGTAMHTVMQHIALPSDEPCDEIRIHQLLDSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFESDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=983293 NSQ25_RS05195 WP_326136607.1 1036357..1040061(+) (addA) [Bacillus sp. FSL R5-0512]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCTAAGAACCCCGGGTCCCTTCATATGAGAAGACAGCTATCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGTTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGTCATGATTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCATCCTTCACCTGAACAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTAGCCCTATCAAAAGAACCGGGTGGCCCCGCGCCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAGATCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGCAGTCCCGCCCAGCATTTGAAAAGTTTAGCTGAAATGAAGCCGGTGATCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATTGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAGGAAACGATTTTAAGGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTAGCTGAGCCTATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGATTACGA
TGAACAAGCTGAATTAAAGCTTGGAGCTAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAACTAGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTACTTCGCTCCATGCCTTGGGCTCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGCTATGCAACATTACCGCTTGTTGCGATGA
AAAAAAAGATGAGGAAAGAGTTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAGAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTCCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCACACCCATCAGCATTCACGATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAGGACGAGGTGGTACAAGCTTTGATGGAAGGTCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATACCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCAGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGACCGT
GCCACGATGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTGCGATGAAATCCGTATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAACAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCGGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAATCGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATATTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGTAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.425

100

0.667

Multiple sequence alignment