Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NST32_RS16705 Genome accession   NZ_CP151978
Coordinates   3208041..3209438 (-) Length   465 a.a.
NCBI ID   WP_217008717.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0215     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3203041..3214438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS16670 (NST32_16670) flgL 3203242..3204150 (-) 909 WP_034281095.1 flagellar hook-associated protein FlgL -
  NST32_RS16675 (NST32_16675) flgK 3204160..3205680 (-) 1521 WP_024423618.1 flagellar hook-associated protein FlgK -
  NST32_RS16680 (NST32_16680) - 3205696..3206178 (-) 483 WP_024423619.1 flagellar protein FlgN -
  NST32_RS16685 (NST32_16685) flgM 3206197..3206460 (-) 264 WP_024423620.1 flagellar biosynthesis anti-sigma factor FlgM -
  NST32_RS16690 (NST32_16690) - 3206540..3206959 (-) 420 WP_034281098.1 TIGR03826 family flagellar region protein -
  NST32_RS16695 (NST32_16695) comFC 3207016..3207672 (-) 657 WP_260983460.1 ComF family protein Machinery gene
  NST32_RS16700 (NST32_16700) - 3207699..3207992 (-) 294 WP_024423623.1 competence protein ComFB -
  NST32_RS16705 (NST32_16705) comFA 3208041..3209438 (-) 1398 WP_217008717.1 DEAD/DEAH box helicase Machinery gene
  NST32_RS16710 (NST32_16710) - 3209559..3210401 (-) 843 WP_214755360.1 DegV family protein -
  NST32_RS16715 (NST32_16715) degU 3210617..3211306 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NST32_RS16720 (NST32_16720) degS 3211371..3212546 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  NST32_RS16725 (NST32_16725) - 3212767..3213435 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52903.74 Da        Isoelectric Point: 9.8335

>NTDB_id=983113 NST32_RS16705 WP_217008717.1 3208041..3209438(-) (comFA) [Bacillus sp. FSL L8-0215]
MNMDKAMELMGQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKAAECGFLYEWTGPQMVETGRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMSVCIATPRTDVVLELEPRLRKAFQGVIITVLYGGSSQRFQIAPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNGGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEHPLPVPTFHWIGHWKKKLKKNQLSPKVMNWMQ
RHIANKRRILLFVPSIAVMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=983113 NST32_RS16705 WP_217008717.1 3208041..3209438(-) (comFA) [Bacillus sp. FSL L8-0215]
ATGAATATGGACAAAGCAATGGAACTAATGGGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTCTATGAATGGACAGGTCCACAAATGGTAGAAACAGG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCATTA
AATCAAGGAATGAGTGTCTGTATTGCGACACCTAGAACAGATGTTGTGCTTGAACTCGAACCGCGGCTTAGAAAAGCATT
TCAAGGGGTGATAATTACCGTACTTTATGGAGGCAGTTCTCAAAGGTTTCAAATAGCACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGACTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACGGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGCGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCACCCATTACCCGTAC
CGACCTTTCATTGGATCGGACATTGGAAAAAGAAATTAAAAAAGAACCAGCTGTCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTGTCATGAAGAAGGTAACGAAGATTCTTCG
GGAACAATCCTTAAACGTGGAGGGAGTATCTGCCGATGATCCGGATAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACCATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGACGGGTAGGCAGACATCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(324-420)

Type III restriction enzyme, res subunit (ResIII)

(116-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.974

98.065

0.51
  comFA/cflA Streptococcus pneumoniae Rx1

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae D39

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae R6

38.095

94.839

0.361

Multiple sequence alignment