Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NST32_RS05525 Genome accession   NZ_CP151978
Coordinates   1074886..1078590 (+) Length   1234 a.a.
NCBI ID   WP_340899042.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0215     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1069886..1083590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS05510 (NST32_05510) - 1070192..1070602 (+) 411 WP_034282155.1 DoxX family protein -
  NST32_RS05515 (NST32_05515) - 1070800..1071246 (-) 447 WP_034282153.1 ferritin-like domain-containing protein -
  NST32_RS05520 (NST32_05520) addB 1071399..1074908 (+) 3510 WP_340899044.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NST32_RS05525 (NST32_05525) addA 1074886..1078590 (+) 3705 WP_340899042.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NST32_RS05530 (NST32_05530) sbcD 1078617..1079792 (+) 1176 WP_070325681.1 exonuclease subunit SbcD -
  NST32_RS05535 (NST32_05535) - 1079789..1083184 (+) 3396 WP_340899041.1 SMC family ATPase -
  NST32_RS05540 (NST32_05540) - 1083204..1083503 (+) 300 WP_034282144.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142365.39 Da        Isoelectric Point: 4.9067

>NTDB_id=983074 NST32_RS05525 WP_340899042.1 1074886..1078590(+) (addA) [Bacillus sp. FSL L8-0215]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQINELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILRLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGEETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIDYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREDLETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKNQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATMYDRPAFMKKKTLTAAEQGTAMHTVMQHIALPSDEPFDEIRIHQLLDSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFESDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAKVVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=983074 NST32_RS05525 WP_340899042.1 1074886..1078590(+) (addA) [Bacillus sp. FSL L8-0215]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCTAAGAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCATTCCTTTTGTTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGTCATGATTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAATT
TTCTAGATCTCATCCTTCACCTGAACAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTTGAACTGCTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGTGGCCCCGCGCCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAGATCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAACTAGAAAAGCTCAAA
GATGAATACTTTATGCGCAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATTGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAGGAAACGATTTTAAGGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCTATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCGAAGAAACAGGGAAAAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGATTACGA
TGAACAAGCTGAATTAAAGCTTGGAGCTAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGATCTAGAGACCGTACAACTAGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTACTTCGCTCCATGCCTTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTAATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGATTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGCTATGCAACATTACCGCTTGTGGCAATGA
AAAAAAAGATGAGGAAAGAGTTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACTGGAGAGGACTGGCTCCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTGTCGCACCCATCAGCATTCACGATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAATCAGCAGGACGAGGTGGTACAAGCTTTGATGGAAGGTCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATACCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCAGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCCCGT
GCCACGATGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTTTGATGAAATCCGTATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAACAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAATCGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATATTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGTAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGTAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.262

100

0.664

Multiple sequence alignment