Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NST32_RS05520 Genome accession   NZ_CP151978
Coordinates   1071399..1074908 (+) Length   1169 a.a.
NCBI ID   WP_340899044.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0215     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1066399..1079908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS05490 (NST32_05490) lepB 1066917..1067429 (+) 513 WP_034282162.1 signal peptidase I -
  NST32_RS05495 (NST32_05495) - 1067468..1068613 (-) 1146 WP_034282161.1 AbrB family transcriptional regulator -
  NST32_RS05500 (NST32_05500) - 1068880..1069302 (+) 423 WP_034282159.1 BlaI/MecI/CopY family transcriptional regulator -
  NST32_RS05505 (NST32_05505) - 1069295..1070134 (+) 840 WP_034282157.1 M56 family metallopeptidase -
  NST32_RS05510 (NST32_05510) - 1070192..1070602 (+) 411 WP_034282155.1 DoxX family protein -
  NST32_RS05515 (NST32_05515) - 1070800..1071246 (-) 447 WP_034282153.1 ferritin-like domain-containing protein -
  NST32_RS05520 (NST32_05520) addB 1071399..1074908 (+) 3510 WP_340899044.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NST32_RS05525 (NST32_05525) addA 1074886..1078590 (+) 3705 WP_340899042.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NST32_RS05530 (NST32_05530) sbcD 1078617..1079792 (+) 1176 WP_070325681.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134804.96 Da        Isoelectric Point: 5.4399

>NTDB_id=983073 NST32_RS05520 WP_340899044.1 1071399..1074908(+) (addB) [Bacillus sp. FSL L8-0215]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTEQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGASISEHILEKNNRHLHTPDLAYLERQYEQRPVKPYQEITPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSGLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGSLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSPLIKYCQELMPNHEHALYVLDPEQLDDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEKEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=983073 NST32_RS05520 WP_340899044.1 1071399..1074908(+) (addB) [Bacillus sp. FSL L8-0215]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATTAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTCGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATATT
GCATGCTGCCTGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACGCTTCTCACGGAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAACAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGCGCGTCCATTTCTGAGCACATTTTGGAGAAAAATAACCGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACGGCAATATGAGCAGAGACCGGTGAAGCCATATCAGGAAATAACCCCTCATCTCACAGTGTCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCAGGCTTGGATGTCATTAAAGGAAACTGGCGTTAT
GAGGCCGTGTTTCGCTGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
CCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGGCGAT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCGCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTAGAGGCAGTTTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCTGAAGAAGCAGGTAGCTTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGTCCTTCTGACGAAAGTGTTTTGTCTGAGGATGACCGGGAGTGGTTAAAACGAGCAGGAGCAGAACTCGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCACTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGCCTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACAAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATACCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTATGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCACCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAC
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGTGGCCAAGGGCCACTTCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGCCTTGATCTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACATACTTAGA
CCTGACGATTACGTATTCAAAGGAGTGGCTCGGTGTAGAAGCAACGCCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATCGAGAAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTT
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCTGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.805

99.316

0.654

Multiple sequence alignment