Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSQ52_RS11500 Genome accession   NZ_CP151973
Coordinates   2254410..2255480 (-) Length   356 a.a.
NCBI ID   WP_342473345.1    Uniprot ID   -
Organism   Bacillus sp. FSL W8-1141     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2249410..2260480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ52_RS11450 (NSQ52_11450) - 2249505..2250077 (-) 573 WP_058336655.1 signal peptidase I -
  NSQ52_RS11455 (NSQ52_11455) tapA 2250127..2250654 (-) 528 WP_058336654.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ52_RS11460 (NSQ52_11460) - 2250907..2251233 (+) 327 WP_058336653.1 DUF3889 domain-containing protein -
  NSQ52_RS11465 (NSQ52_11465) - 2251268..2251462 (-) 195 WP_024718846.1 YqzE family protein -
  NSQ52_RS11470 (NSQ52_11470) comGG 2251523..2251906 (-) 384 WP_058336652.1 competence type IV pilus minor pilin ComGG -
  NSQ52_RS11475 (NSQ52_11475) comGF 2251903..2252400 (-) 498 WP_039166031.1 competence type IV pilus minor pilin ComGF -
  NSQ52_RS11480 (NSQ52_11480) comGE 2252342..2252656 (-) 315 WP_007501227.1 competence type IV pilus minor pilin ComGE -
  NSQ52_RS11485 (NSQ52_11485) comGD 2252640..2253086 (-) 447 WP_058336650.1 competence type IV pilus minor pilin ComGD -
  NSQ52_RS11490 (NSQ52_11490) comGC 2253079..2253372 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NSQ52_RS11495 (NSQ52_11495) comGB 2253389..2254429 (-) 1041 WP_058336649.1 competence type IV pilus assembly protein ComGB -
  NSQ52_RS11500 (NSQ52_11500) comGA 2254410..2255480 (-) 1071 WP_342473345.1 competence type IV pilus ATPase ComGA Machinery gene
  NSQ52_RS11505 (NSQ52_11505) - 2255937..2256809 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NSQ52_RS11515 (NSQ52_11515) - 2257035..2257415 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NSQ52_RS11520 (NSQ52_11520) - 2257628..2257870 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NSQ52_RS11525 (NSQ52_11525) - 2257919..2258557 (-) 639 WP_342473346.1 MBL fold metallo-hydrolase -
  NSQ52_RS11530 (NSQ52_11530) - 2258718..2258891 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NSQ52_RS11535 (NSQ52_11535) - 2258916..2259230 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40394.72 Da        Isoelectric Point: 9.0449

>NTDB_id=982792 NSQ52_RS11500 WP_342473345.1 2254410..2255480(-) (comGA) [Bacillus sp. FSL W8-1141]
MYGIEYLGQELLEEACRMRTSDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTVNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=982792 NSQ52_RS11500 WP_342473345.1 2254410..2255480(-) (comGA) [Bacillus sp. FSL W8-1141]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAACATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAAGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGGTGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.045

100

0.64

Multiple sequence alignment