Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MHI30_RS05915 Genome accession   NZ_CP151961
Coordinates   1179454..1183146 (+) Length   1230 a.a.
NCBI ID   WP_075754891.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1174454..1188146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS05905 (MHI30_05905) modB 1175201..1175857 (+) 657 WP_075754887.1 molybdate ABC transporter permease subunit -
  MHI30_RS05910 (MHI30_05910) addB 1175967..1179467 (+) 3501 WP_075754889.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHI30_RS05915 (MHI30_05915) addA 1179454..1183146 (+) 3693 WP_075754891.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHI30_RS05920 (MHI30_05920) sbcD 1183213..1184394 (+) 1182 WP_075754893.1 exonuclease subunit SbcD -
  MHI30_RS05925 (MHI30_05925) sbcC 1184391..1187783 (+) 3393 WP_075754895.1 exonuclease subunit SbcC -
  MHI30_RS05930 (MHI30_05930) - 1187828..1188046 (-) 219 WP_016885923.1 spore germination protein -

Sequence

Protein


Download         Length: 1230 a.a.        Molecular weight: 141306.06 Da        Isoelectric Point: 5.3781

>NTDB_id=982240 MHI30_RS05915 WP_075754891.1 1179454..1183146(+) (addA) [Bacillus sp. FSL L8-0528]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQLEKLK
SDYFTRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDEGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLFIENQSGAPDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLSAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPQVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPTKEAL
LFNRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEINELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide


Download         Length: 3693 bp        

>NTDB_id=982240 MHI30_RS05915 WP_075754891.1 1179454..1183146(+) (addA) [Bacillus sp. FSL L8-0528]
ATGGAGATTTCTAAACCGAAGGGCAGCAACTGGACTGATGACCAATGGAAGGCGATCGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACGTC
CCGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTTGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTTGTCAAGCGGGTCTACGAGTT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACACATTTGTTGATCTGTATGACGCTGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAGGGGATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAGCAGCCCGGGGGACCTGCTCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCCCTGTACGAGCTTGTGCCTGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGCTTGGTTGATGAGGCGGCAGACCTCCGCAACAGCGCGAAAAAGCAACTTGAAAAGCTGAAA
AGCGATTATTTTACGCGAACTCCCGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTAGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCATGAAGTGCTTGTTGATGAATATCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGATGAAGGCTCTGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTTAGAAGCCGT
TCAGATATTTTGGACAGTACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
AGCAGAATTGAAGCTTGGCGCTTCTTACCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGTTCATCGAAAACCAGAGCG
GCGCGCCGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAAATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACAG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGACGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAGCATACCTTTCTGCAGCAGACGGCGATGAAAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATTTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGGTTTATTGAGCGGATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAGTCTTATCTGCTGGATAAAGAACTTGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGACACTGCCGCTAATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCTCTTACTCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCCGGGTCTGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCAGGTGATTGATGAGATCCGCGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCAGATCTTCAAGCAGAG
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGGCTTCCGATCGACGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAACGACTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAATATGAGGATGAGTACGGCGACCGCTCGCTGATTCGCCCAACCAAGGAGGCTCTC
CTGTTTAATCGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCGGCCGAACGGGGAACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAATAAATGAGCTCAGCCGTCTCCTTGACAGACTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTACCCGGATCTCGAGTCAGCCGA
CGAACCTGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCG
ACCGGATACACGGAAAGTACCGAAACGGCTTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAG
CTTTATGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1051-1222)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-460)

UvrD-like helicase C-terminal domain (UvrD_C)

(471-880)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

72.136

100

0.722

Multiple sequence alignment