Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHI30_RS05910 Genome accession   NZ_CP151961
Coordinates   1175967..1179467 (+) Length   1166 a.a.
NCBI ID   WP_075754889.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1170967..1184467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS05885 (MHI30_05885) - 1171624..1172991 (-) 1368 WP_048350705.1 HAMP domain-containing sensor histidine kinase -
  MHI30_RS05890 (MHI30_05890) - 1172988..1173662 (-) 675 WP_075754883.1 response regulator transcription factor -
  MHI30_RS05895 (MHI30_05895) - 1173753..1174193 (-) 441 WP_048350704.1 ferritin-like domain-containing protein -
  MHI30_RS05900 (MHI30_05900) modA 1174398..1175192 (+) 795 WP_075754885.1 molybdate ABC transporter substrate-binding protein -
  MHI30_RS05905 (MHI30_05905) modB 1175201..1175857 (+) 657 WP_075754887.1 molybdate ABC transporter permease subunit -
  MHI30_RS05910 (MHI30_05910) addB 1175967..1179467 (+) 3501 WP_075754889.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHI30_RS05915 (MHI30_05915) addA 1179454..1183146 (+) 3693 WP_075754891.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHI30_RS05920 (MHI30_05920) sbcD 1183213..1184394 (+) 1182 WP_075754893.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134563.69 Da        Isoelectric Point: 5.6399

>NTDB_id=982239 MHI30_RS05910 WP_075754889.1 1175967..1179467(+) (addB) [Bacillus sp. FSL L8-0528]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLPPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYEDEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADKP
FTANSPNELHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHQQHPELRHLERYFDERPAKPYRGRTESLRIMQASNR
RTEIEGIAREIHSLIRKGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPVHRLQKRLKKAETVREMAEAVYLYLEEANVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
AALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLQELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNMISAGLKKDGSLRSDSDVIAEEDFHVLRRHIRRTFQKAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDNVILERLKKEDETDGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=982239 MHI30_RS05910 WP_075754889.1 1175967..1179467(+) (addB) [Bacillus sp. FSL L8-0528]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGTACCATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTTCAGCACACCGGA
GGCATGAACCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTTCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAAGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCCGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCTGAT
AAATTGCACGACTTATATATTTTGTACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGAGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCACAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCGGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCCAACAGCCCGAATGAGCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAGAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAGACGAAACGGCACCAGCAACATCCGGAGCTCCGGCACCTTG
AACGCTATTTTGACGAGCGTCCTGCGAAACCGTATCGCGGACGGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCAAGGGCCGGTTCCGGCTTAGGGATATCGCCGT
CATCGCCCGCAATGTCGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATTCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGTTGTGTCAAAACGGAGCTTTTATTCCCGGAGGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGAT
ACGCTTCACTGGATGAGGATTTCGCCAAGACAGATAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCGCCGGTCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTCTACCT
TTATCTTGAAGAAGCCAATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCCGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACCGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGCATAC
TACCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACAGAGAGTGGCTGAAACGAAACGGCGCACAGCTGGCG
GCGACGGGGCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTACATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTGGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGATGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
GCCGCCTTGAGCCATTTGGCAGGACAGCTCGGCATCTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAACCTGACCGCATTTTTTCTCAAAATATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACTTGAACCGGCATGTGAGCCGCGATTTATATGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAAGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGTTTAAAGCTGAAGGAACGCCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCGGCGCTGAAGCTGATTTCAGACCGGCTTCAAGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAGGAGATTCTGCTCAGTTCGAAC
CGTCATCATTATGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGTGAGCATGCAAAAGCAAG
CGGCTTTGCCCCCGTCGGCATAGAGCTCGGATTCGGGGGGAAAGGTCCGCTTCCTCCAATGCGTTTTACCTTGAAGAACG
GCTGCACGATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTC
GACTATAAATCAAGTGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGCCTCGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACGCATTCAACCGATTGGCTCGGCATGAAGGCATCCCCGGCAGGCGTGCTTTATTTCCATGTGCACGATC
CGATGATCCAGGCTTCGGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCTTGGGAGACCAGGAAGCGGTCAAGCTGATGGATACCACACTGGAACAAGGCAGGTCGAACATGATCAGCGCCGGTTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTATTGCAGAAGAGGATTTTCATGTGTTGAGGCGCCATATCCGCC
GGACATTTCAGAAGGCCGGCGAGGAGATCACAGACGGCAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCATACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGGATATTAAAACCCGA
AAAAGACAACGTGATTTTGGAGCGGCTGAAAAAGGAGGATGAGACAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.82

99.914

0.738

Multiple sequence alignment