Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI30_RS02035 Genome accession   NZ_CP151961
Coordinates   381421..381867 (-) Length   148 a.a.
NCBI ID   WP_075753823.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 376421..386867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS02010 (MHI30_02010) - 377027..377902 (-) 876 WP_075753813.1 transporter substrate-binding domain-containing protein -
  MHI30_RS02015 (MHI30_02015) - 378086..378637 (+) 552 WP_075753815.1 NAD(P)H-dependent oxidoreductase -
  MHI30_RS02020 (MHI30_02020) - 378733..379458 (+) 726 WP_075753817.1 AAA family ATPase -
  MHI30_RS02025 (MHI30_02025) - 379528..380964 (+) 1437 WP_075753819.1 family 1 glycosylhydrolase -
  MHI30_RS02030 (MHI30_02030) nin/comJ 381005..381403 (-) 399 WP_075753821.1 competence protein ComJ Regulator
  MHI30_RS02035 (MHI30_02035) nucA/comI 381421..381867 (-) 447 WP_075753823.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI30_RS02040 (MHI30_02040) - 382135..382338 (+) 204 WP_128814219.1 hypothetical protein -
  MHI30_RS02045 (MHI30_02045) - 382344..383147 (+) 804 WP_075753825.1 protein kinase -
  MHI30_RS02050 (MHI30_02050) - 383534..384274 (-) 741 WP_075753827.1 LXG domain-containing protein -
  MHI30_RS02055 (MHI30_02055) - 384679..386733 (+) 2055 WP_075753829.1 hydantoinase/oxoprolinase family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16307.39 Da        Isoelectric Point: 6.2413

>NTDB_id=982230 MHI30_RS02035 WP_075753823.1 381421..381867(-) (nucA/comI) [Bacillus sp. FSL L8-0528]
MDKIKSLLITLLAVAVVIIGIISGDFFTSEHQPSGRQNGSDYDEVLIFPSDRYPETGAHIRKAIKKGHSKICTIDRDGAD
ERRKESLKDVPSKSGYDRDEWPMAMCEEGGKGASVEYISPSDNRGAGSWVGNQVSDYPDGTKILFKIK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=982230 MHI30_RS02035 WP_075753823.1 381421..381867(-) (nucA/comI) [Bacillus sp. FSL L8-0528]
ATGGACAAAATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGCATTATATCCGGCGATTTTTT
CACTTCAGAACACCAGCCTTCAGGCCGGCAAAACGGCTCAGACTATGATGAAGTGCTGATTTTTCCGTCGGACCGCTATC
CTGAAACAGGCGCGCACATTCGAAAGGCCATCAAGAAAGGGCATTCCAAAATCTGTACGATCGACAGGGACGGAGCCGAC
GAACGCCGAAAGGAATCGTTAAAAGATGTTCCTTCCAAATCGGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAAAAGGTGCTTCCGTCGAGTATATCAGTCCTTCTGATAACCGCGGAGCAGGATCGTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGCACGAAAATACTTTTCAAGATTAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.946

99.324

0.635

Multiple sequence alignment