Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI42_RS29395 Genome accession   NZ_CP151953
Coordinates   6763284..6763724 (-) Length   146 a.a.
NCBI ID   WP_342421442.1    Uniprot ID   -
Organism   Paenibacillus sp. FSL E2-0178     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 6758284..6768724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI42_RS29345 (MHI42_29345) - 6758391..6758750 (+) 360 WP_342421437.1 MerR family transcriptional regulator -
  MHI42_RS29350 (MHI42_29350) - 6758849..6759118 (+) 270 WP_171719978.1 hypothetical protein -
  MHI42_RS29355 (MHI42_29355) - 6759235..6759432 (+) 198 WP_342421438.1 hypothetical protein -
  MHI42_RS29360 (MHI42_29360) tnpA 6759830..6760228 (+) 399 WP_342421439.1 IS200/IS605 family transposase -
  MHI42_RS29365 (MHI42_29365) tnpB 6760238..6761311 (+) 1074 WP_342421440.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  MHI42_RS29370 (MHI42_29370) - 6761412..6761576 (-) 165 WP_342421441.1 hypothetical protein -
  MHI42_RS29375 (MHI42_29375) - 6761664..6762206 (-) 543 WP_171719980.1 ImmA/IrrE family metallo-endopeptidase -
  MHI42_RS29380 (MHI42_29380) - 6762350..6762526 (-) 177 WP_171719981.1 YjfB family protein -
  MHI42_RS29385 (MHI42_29385) - 6762701..6762856 (+) 156 WP_155991357.1 hypothetical protein -
  MHI42_RS29390 (MHI42_29390) - 6762905..6763042 (-) 138 WP_156118708.1 hypothetical protein -
  MHI42_RS29395 (MHI42_29395) nucA/comI 6763284..6763724 (-) 441 WP_342421442.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MHI42_RS29400 (MHI42_29400) - 6763953..6765416 (+) 1464 WP_342421443.1 glycosyl hydrolase -
  MHI42_RS29405 (MHI42_29405) - 6765434..6765931 (-) 498 WP_342421444.1 PH domain-containing protein -
  MHI42_RS29410 (MHI42_29410) - 6766160..6766750 (+) 591 WP_342421445.1 hypothetical protein -
  MHI42_RS29415 (MHI42_29415) - 6766994..6767329 (+) 336 WP_342421446.1 YolD-like family protein -
  MHI42_RS29420 (MHI42_29420) - 6767366..6767527 (-) 162 WP_342421447.1 hypothetical protein -
  MHI42_RS29425 (MHI42_29425) - 6767779..6768465 (+) 687 WP_342421448.1 M15 family metallopeptidase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16243.09 Da        Isoelectric Point: 4.3703

>NTDB_id=981967 MHI42_RS29395 WP_342421442.1 6763284..6763724(-) (nucA/comI) [Paenibacillus sp. FSL E2-0178]
MKAKKFITSLIIVVLLSLAAYYFGENGQLPSQTPSSDNSEVVRLEFPSDRYPETARHIQEAIENGESPICTIDRNEAEEN
RKESLKGVPTKKGYDRDEWPMAMCAEGGAGADIEYITPSDNRGAGSWVGNQLEDFADGTRVEFMFK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=981967 MHI42_RS29395 WP_342421442.1 6763284..6763724(-) (nucA/comI) [Paenibacillus sp. FSL E2-0178]
ATGAAAGCCAAAAAATTCATCACCAGTCTCATTATTGTTGTTCTGCTCAGCCTGGCTGCGTACTACTTCGGGGAGAATGG
TCAGCTCCCGTCTCAGACGCCGTCTTCTGACAATTCGGAGGTTGTCCGGCTGGAATTCCCGTCTGACCGTTATCCGGAGA
CCGCCAGACATATTCAGGAAGCGATTGAGAACGGGGAGTCCCCCATCTGCACGATTGACCGGAATGAAGCCGAAGAGAAC
CGTAAGGAATCCCTGAAGGGCGTACCCACCAAAAAAGGTTATGACCGCGATGAATGGCCGATGGCGATGTGTGCAGAAGG
CGGAGCCGGAGCCGATATTGAATACATAACCCCAAGTGATAACCGCGGGGCAGGAAGCTGGGTAGGCAATCAGCTGGAGG
ACTTCGCAGACGGTACCCGCGTAGAGTTCATGTTCAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.025

81.507

0.514

Multiple sequence alignment