Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACNR0F_RS02315 Genome accession   NZ_CP183037
Coordinates   419992..420651 (+) Length   219 a.a.
NCBI ID   WP_003785824.1    Uniprot ID   F5S5N3
Organism   Kingella kingae strain URMC_2412A945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414992..425651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR0F_RS02300 (ACNR0F_02300) serB 415465..416301 (+) 837 WP_418605037.1 phosphoserine phosphatase SerB -
  ACNR0F_RS02305 (ACNR0F_02305) - 416376..418745 (-) 2370 WP_285052202.1 penicillin-binding protein 1A -
  ACNR0F_RS02310 (ACNR0F_02310) pilM 418887..419990 (+) 1104 WP_003785822.1 type IV pilus biogenesis protein PilM -
  ACNR0F_RS02315 (ACNR0F_02315) pilN 419992..420651 (+) 660 WP_003785824.1 PilN domain-containing protein Machinery gene
  ACNR0F_RS02320 (ACNR0F_02320) - 420654..421277 (+) 624 WP_003785825.1 type 4a pilus biogenesis protein PilO -
  ACNR0F_RS02325 (ACNR0F_02325) pilP 421311..421646 (+) 336 WP_418605039.1 pilus assembly protein PilP Machinery gene
  ACNR0F_RS02330 (ACNR0F_02330) pilP 421639..421842 (+) 204 WP_418605040.1 pilus assembly protein PilP Machinery gene
  ACNR0F_RS02335 (ACNR0F_02335) pilQ 421876..423978 (+) 2103 WP_003789799.1 type IV pilus secretin PilQ Machinery gene
  ACNR0F_RS02340 (ACNR0F_02340) - 424035..424547 (+) 513 WP_003789800.1 shikimate kinase -
  ACNR0F_RS02345 (ACNR0F_02345) aroB 424547..425629 (+) 1083 WP_418605041.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 23543.16 Da        Isoelectric Point: 9.5918

>NTDB_id=981452 ACNR0F_RS02315 WP_003785824.1 419992..420651(+) (pilN) [Kingella kingae strain URMC_2412A945]
MNLIKINLLPYREMEEQKKKKQFRTIMVIGAAAGLVAAGLIYSSLAGMILNQQGRNESLQAGIATLDKELEEIKTLNEQK
RNFLERRQRIAELDNKRFEGARIIDTLNQLVPDGAYLLSLKGDASKNLVSNKYAITGKAISDNKVAVLMTALPSTGIFEQ
PELVKIEKTDDGQQFILNALLLEQKVIVPDVVGGGASVSSGAAAASVTSTPATSASGGK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=981452 ACNR0F_RS02315 WP_003785824.1 419992..420651(+) (pilN) [Kingella kingae strain URMC_2412A945]
ATGAATTTGATTAAGATTAACTTGCTTCCCTATCGTGAGATGGAAGAGCAAAAGAAAAAGAAACAATTTCGAACAATCAT
GGTAATTGGTGCAGCAGCAGGTTTGGTTGCTGCGGGTCTGATTTACTCATCATTGGCTGGTATGATACTGAATCAGCAGG
GGCGTAACGAAAGTTTGCAGGCTGGTATTGCCACGCTAGATAAAGAATTGGAAGAAATTAAAACGCTCAATGAGCAAAAA
CGTAATTTTTTGGAACGTCGTCAACGTATCGCAGAATTGGATAATAAGCGATTTGAGGGTGCGCGAATTATTGATACATT
AAATCAACTTGTTCCCGACGGTGCTTATTTGTTGTCTTTGAAAGGCGATGCTTCTAAGAATTTGGTAAGTAATAAGTATG
CTATTACTGGTAAAGCGATTAGCGATAACAAAGTTGCTGTTTTAATGACTGCTTTACCAAGTACGGGTATTTTTGAACAG
CCAGAATTGGTGAAAATTGAAAAAACCGATGATGGTCAACAATTTATCTTGAATGCATTATTATTGGAACAAAAAGTGAT
TGTTCCTGATGTGGTGGGTGGTGGTGCATCTGTTTCATCAGGTGCAGCAGCTGCGTCGGTAACATCAACACCTGCTACAA
GTGCATCGGGAGGTAAATAA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S5N3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Neisseria meningitidis strain C311

45.556

82.192

0.374

Multiple sequence alignment