Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Regulator
Locus tag   WLF14_RS28825 Genome accession   NZ_CP151816
Coordinates   6324091..6324498 (-) Length   135 a.a.
NCBI ID   WP_010564379.1    Uniprot ID   A0A7Y8D5M8
Organism   Pseudomonas fluorescens strain IMGN2     
Function   regulation of type IV pilus assembly (predicted from homology)   
Competence regulation

Genomic Context

Location: 6319091..6329498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS28810 (WLF14_28810) - 6321094..6323145 (-) 2052 WP_024077895.1 methyl-accepting chemotaxis protein -
  WLF14_RS28815 (WLF14_28815) - 6323142..6323666 (-) 525 WP_024077896.1 chemotaxis protein CheW -
  WLF14_RS28820 (WLF14_28820) pilH 6323679..6324044 (-) 366 WP_014720320.1 twitching motility response regulator PilH -
  WLF14_RS28825 (WLF14_28825) pilG 6324091..6324498 (-) 408 WP_010564379.1 twitching motility response regulator PilG Regulator
  WLF14_RS28830 (WLF14_28830) gshB 6324738..6325691 (+) 954 WP_024077897.1 glutathione synthase -
  WLF14_RS28835 (WLF14_28835) - 6325769..6326674 (+) 906 WP_024077898.1 energy transducer TonB -
  WLF14_RS28840 (WLF14_28840) - 6326756..6327325 (+) 570 WP_024077899.1 YqgE/AlgH family protein -
  WLF14_RS28845 (WLF14_28845) ruvX 6327325..6327762 (+) 438 WP_342308500.1 Holliday junction resolvase RuvX -
  WLF14_RS28850 (WLF14_28850) pyrR 6327846..6328352 (+) 507 WP_024077900.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  WLF14_RS28855 (WLF14_28855) - 6328377..6329381 (+) 1005 WP_024077901.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14906.40 Da        Isoelectric Point: 7.3842

>NTDB_id=981011 WLF14_RS28825 WP_010564379.1 6324091..6324498(-) (pilG) [Pseudomonas fluorescens strain IMGN2]
MEQHSNALRVMVIDDSKTIRRTAETLLKNVGCDVITAIDGFDALARIVDHHPHIIFVDIMMPRLDGYQTCALVKNNPAFK
SIPVIMLSSKDGLFDKAKGRIVGVDQFLTKPFSKEELLGAIKAYVPGFAAVEQAH

Nucleotide


Download         Length: 408 bp        

>NTDB_id=981011 WLF14_RS28825 WP_010564379.1 6324091..6324498(-) (pilG) [Pseudomonas fluorescens strain IMGN2]
ATGGAACAGCATTCCAACGCCTTGAGAGTGATGGTGATCGACGATTCGAAAACGATCCGCCGCACCGCCGAAACCTTGTT
GAAGAATGTGGGATGCGATGTCATCACGGCCATCGACGGTTTCGATGCCCTGGCTCGGATTGTGGATCATCACCCGCACA
TTATCTTTGTCGACATCATGATGCCGCGCCTGGATGGCTATCAGACCTGCGCCCTGGTGAAGAACAACCCGGCGTTCAAG
TCGATCCCGGTGATCATGCTCTCCTCCAAGGACGGCCTGTTCGACAAGGCCAAGGGGCGCATCGTCGGAGTTGATCAGTT
TTTGACCAAGCCTTTCAGCAAGGAAGAACTGCTGGGCGCGATCAAGGCCTACGTGCCTGGGTTCGCCGCAGTAGAACAAG
CACACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(10-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8D5M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Acinetobacter baumannii strain A118

70.4

92.593

0.652
  vicR Streptococcus mutans UA159

38.889

93.333

0.363

Multiple sequence alignment