Detailed information NTG0980799

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAFA31_RS22660
• Genome accession: NZ_CP151768
• Coordinates: 4692404..4693201 (+)
• Length: 265 a.a.
• NCBI ID: WP_004098693.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain 2024HQ-00017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4687404..4698201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA31_RS22625 (AAFA31_22630)	gspH	4687545..4688057 (+)	513	WP_004098708.1	type II secretion system minor pseudopilin GspH	-
AAFA31_RS22630 (AAFA31_22635)	gspI	4688054..4688419 (+)	366	WP_004098707.1	type II secretion system minor pseudopilin GspI	-
AAFA31_RS22635 (AAFA31_22640)	gspJ	4688416..4689012 (+)	597	WP_004098705.1	type II secretion system minor pseudopilin GspJ	-
AAFA31_RS22640 (AAFA31_22645)	gspK	4689009..4689989 (+)	981	WP_004098702.1	type II secretion system minor pseudopilin GspK	-
AAFA31_RS22645 (AAFA31_22650)	gspL	4689982..4691175 (+)	1194	WP_004098701.1	type II secretion system protein GspL	-
AAFA31_RS22650 (AAFA31_22655)	-	4691168..4691653 (+)	486	WP_004098698.1	type II secretion system protein M	-
AAFA31_RS22655 (AAFA31_22660)	-	4691643..4692401 (+)	759	WP_004098694.1	type II secretion system protein N	-
AAFA31_RS22660 (AAFA31_22665)	pilD	4692404..4693201 (+)	798	WP_004098693.1	A24 family peptidase	Machinery gene
AAFA31_RS22665 (AAFA31_22670)	hrpB	4693299..4695728 (-)	2430	WP_004098692.1	ATP-dependent helicase HrpB	-
AAFA31_RS22670 (AAFA31_22675)	thpR	4695801..4696337 (+)	537	WP_004098691.1	RNA 2',3'-cyclic phosphodiesterase	-
AAFA31_RS22675 (AAFA31_22680)	sfsA	4696337..4697053 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
AAFA31_RS22680 (AAFA31_22685)	dksA	4697218..4697673 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29114.76 Da
• Isoelectric Point: 8.0074

>NTDB_id=980799 AAFA31_RS22660 WP_004098693.1 4692404..4693201(+) (pilD) [Klebsiella michiganensis strain 2024HQ-00017]
METIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=980799 AAFA31_RS22660 WP_004098693.1 4692404..4693201(+) (pilD) [Klebsiella michiganensis strain 2024HQ-00017]
ATGGAAACTATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGTGCGCGCTGCTGCGGGCAGCCGATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGGCTAATTTTCGTATCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGTTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCTGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.077	100	0.502
pilD	Neisseria gonorrhoeae MS11	47.826	95.472	0.457
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	45.56	97.736	0.445
pilD	Acinetobacter baumannii D1279779	45.174	97.736	0.442