Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAFA21_RS17245 Genome accession   NZ_CP151709
Coordinates   3601032..3601772 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain F16-05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596032..3606772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFA21_RS17225 (AAFA21_17225) aroB 3596574..3597656 (-) 1083 WP_001194249.1 3-dehydroquinate synthase -
  AAFA21_RS17230 (AAFA21_17230) aroK 3597685..3598254 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AAFA21_RS17235 (AAFA21_17235) pilQ 3598266..3600431 (-) 2166 WP_001017029.1 type IV pilus secretin PilQ family protein Machinery gene
  AAFA21_RS17240 (AAFA21_17240) pilP 3600494..3601021 (-) 528 WP_000695061.1 pilus assembly protein PilP Machinery gene
  AAFA21_RS17245 (AAFA21_17245) pilO 3601032..3601772 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFA21_RS17250 (AAFA21_17250) pilN 3601769..3602410 (-) 642 WP_000201221.1 PilN domain-containing protein Machinery gene
  AAFA21_RS17255 (AAFA21_17255) pilM 3602410..3603468 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  AAFA21_RS17260 (AAFA21_17260) ponA 3603630..3606185 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=980425 AAFA21_RS17245 WP_000076101.1 3601032..3601772(-) (pilO) [Acinetobacter baumannii strain F16-05]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=980425 AAFA21_RS17245 WP_000076101.1 3601032..3601772(-) (pilO) [Acinetobacter baumannii strain F16-05]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACTGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment