Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAFA28_RS02365 Genome accession   NZ_CP151699
Coordinates   495413..496153 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain F19-10-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490413..501153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFA28_RS02350 (AAFA28_02350) ponA 491000..493555 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  AAFA28_RS02355 (AAFA28_02355) pilM 493717..494775 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  AAFA28_RS02360 (AAFA28_02360) pilN 494775..495416 (+) 642 WP_000201221.1 PilN domain-containing protein Machinery gene
  AAFA28_RS02365 (AAFA28_02365) pilO 495413..496153 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFA28_RS02370 (AAFA28_02370) pilP 496164..496691 (+) 528 WP_000695061.1 pilus assembly protein PilP Machinery gene
  AAFA28_RS02375 (AAFA28_02375) pilQ 496754..498919 (+) 2166 WP_001017029.1 type IV pilus secretin PilQ family protein Machinery gene
  AAFA28_RS02380 (AAFA28_02380) aroK 498931..499500 (+) 570 WP_000643712.1 shikimate kinase AroK -
  AAFA28_RS02385 (AAFA28_02385) aroB 499529..500611 (+) 1083 WP_001194249.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=980113 AAFA28_RS02365 WP_000076101.1 495413..496153(+) (pilO) [Acinetobacter baumannii strain F19-10-9]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=980113 AAFA28_RS02365 WP_000076101.1 495413..496153(+) (pilO) [Acinetobacter baumannii strain F19-10-9]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACTGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment