Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AADZ70_RS01030 Genome accession   NZ_CP151679
Coordinates   186623..190327 (+) Length   1234 a.a.
NCBI ID   WP_342008035.1    Uniprot ID   -
Organism   Bacillus sp. YBsi01     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 181623..195327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADZ70_RS01015 (AADZ70_01015) - 181703..182212 (-) 510 WP_016938293.1 ferritin-like domain-containing protein -
  AADZ70_RS01020 (AADZ70_01020) - 182281..182994 (-) 714 WP_342007562.1 DUF421 domain-containing protein -
  AADZ70_RS01025 (AADZ70_01025) addB 183136..186636 (+) 3501 WP_342007563.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AADZ70_RS01030 (AADZ70_01030) addA 186623..190327 (+) 3705 WP_342008035.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AADZ70_RS01035 (AADZ70_01035) sbcD 190393..191565 (+) 1173 WP_047476740.1 exonuclease subunit SbcD -
  AADZ70_RS01040 (AADZ70_01040) sbcC 191562..194954 (+) 3393 WP_342007564.1 exonuclease subunit SbcC -
  AADZ70_RS01045 (AADZ70_01045) - 194971..195270 (+) 300 WP_045925296.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141347.67 Da        Isoelectric Point: 5.2202

>NTDB_id=979659 AADZ70_RS01030 WP_342008035.1 186623..190327(+) (addA) [Bacillus sp. YBsi01]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYDYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFEALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLTDMNPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAADEDGRRVPSEAAVYYQEQ
FREVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNRNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAADTELLLIDSADDADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDELYQKLRTFYDNLQKWRSFSTNHSVAELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNREKQLAKWQAQADRADWLLSEFDRYQATSYLDFIGPALIRHRDMEAHRPPGLSAPEDIAHDPSRFHIRMLHQSE
LLEDNPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLQWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRTLHEKELLTEEQRDAIDIDDIIQFFETEIGK
DLLGARRIDREVPFSMALPAGEVYKDAKTAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAIEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=979659 AADZ70_RS01030 WP_342008035.1 186623..190327(+) (addA) [Bacillus sp. YBsi01]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAGACCGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTCCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACACTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGCTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTCCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATTTGGATCTTCAAGATCTCGTCAAGCGGGTGTACGACTA
TTCACGGTCTCATCCTGATCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAGCAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGCGCTGATAATTTTCTGGATGATCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTGAGGCGCTGTATGAACGGGTTCCCGCGGTCACATTTAAGCGGGCTAAGGCGGTAAAAG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCGACCGATTTGCGAAACGGCGCGAAAAAACTGATCGAAAAATTAAAG
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAACAGACATGAACCCTGTCATTGAAACGCTCGTTCA
GCTCGTGATTTCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCGGCTGATGAAGACGGCCGGCGGGTGCCGAGTGAAGCGGCAGTTTATTATCAAGAGCAG
TTCCGTGAGGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCTATTCTGCAGCTTGTGAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGATGTCAAACAATCGATCTACCGATTCAGACTTGCCGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCGAAACTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGTGCGTCGTATCCGCCGAATGACGCGGCGGATACGGAACTGCTGCTGATTGACAGTGCTG
ATGATGCAGACAGCTCCGAGGATGCCGAAGATTTTGAAACCGTGCATTGGGAAGCAAAAGCGATAGCCGGGGAGATCCGG
AAGCTTGTTTCATCTCCGTTTAAGGTCTATGACGGCAAAACCAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGTTCCATGCCATGGGCGCCGCAATTGATGGAAGAACTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCAGGTTATTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGACAACCCGTATCAGGATATT
CCGCTTGCTTCCGTACTGCGCTCGCCTATTGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTGGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGGTGACAGACATGACGAGCTGTATCAAAAGCTGAGAACAT
TTTATGACAATCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCGGTAGCTGAGCTGATTTGGGAAGTATACCGCGAT
ACAGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATTCACAGCAGCAAAGGGCTTGAA
TTCCCTGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGAACAAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTCCCACTTGTGGCGATGAAGAAAA
AAATGCGCAGAGAGCTCTTATCAGAAGAATTGCGCGTGTTATATGTGGCGCTGACAAGAGCGAAGGAAAAGCTGTTTCTC
ATCGGTTCCTGCAAAAACAGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCGGACAGGGCCGATTGGCTTCTCTCTGA
ATTCGACCGCTATCAGGCGACATCTTATTTGGACTTTATCGGGCCGGCGCTTATCCGCCACCGTGACATGGAGGCGCACA
GGCCGCCGGGTCTTTCGGCTCCGGAGGATATCGCGCACGACCCGTCCCGTTTTCATATCCGGATGCTGCATCAAAGTGAA
CTGCTGGAAGATAACCCAAAAGAACGGGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTCCTTCAATGGGAATACCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCCCTCATCAAACCGTCA
GGGGACACGCTTTTATACAGACGTCCCGGTTTTATGATGAAAAAGGGGCTGACGGCCGCGGAAAAAGGGACGGCGATGCA
TACAGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTTCGGACGCTTCACGAAA
AAGAGCTGCTGACAGAAGAGCAGCGGGACGCTATTGATATCGACGACATTATACAGTTTTTCGAAACTGAAATCGGGAAA
GACCTTCTCGGGGCGCGGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
AAAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGACTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGATTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.415

100

0.824

Multiple sequence alignment