Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AADZ70_RS01025 Genome accession   NZ_CP151679
Coordinates   183136..186636 (+) Length   1166 a.a.
NCBI ID   WP_342007563.1    Uniprot ID   -
Organism   Bacillus sp. YBsi01     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 178136..191636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADZ70_RS01000 (AADZ70_01000) - 178335..179492 (-) 1158 WP_269775514.1 AbrB family transcriptional regulator -
  AADZ70_RS01005 (AADZ70_01005) - 179658..180854 (-) 1197 WP_342007561.1 MFS transporter -
  AADZ70_RS01010 (AADZ70_01010) - 180885..181493 (-) 609 WP_264819952.1 TetR/AcrR family transcriptional regulator -
  AADZ70_RS01015 (AADZ70_01015) - 181703..182212 (-) 510 WP_016938293.1 ferritin-like domain-containing protein -
  AADZ70_RS01020 (AADZ70_01020) - 182281..182994 (-) 714 WP_342007562.1 DUF421 domain-containing protein -
  AADZ70_RS01025 (AADZ70_01025) addB 183136..186636 (+) 3501 WP_342007563.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AADZ70_RS01030 (AADZ70_01030) addA 186623..190327 (+) 3705 WP_342008035.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AADZ70_RS01035 (AADZ70_01035) sbcD 190393..191565 (+) 1173 WP_047476740.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133496.10 Da        Isoelectric Point: 5.4023

>NTDB_id=979658 AADZ70_RS01025 WP_342007563.1 183136..186636(+) (addB) [Bacillus sp. YBsi01]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SGEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAAYTGRQEAFTVIQAQNR
RAELEGIAREIQSLVRDGGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPVQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETREW
MAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMIETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLRAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGNGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=979658 AADZ70_RS01025 WP_342007563.1 183136..186636(+) (addB) [Bacillus sp. YBsi01]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCAATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGA
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTGTTGAGGAAGCTGATTGAAGAGCATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGTTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATCTGCATTCAGAGGATTATTT
GACGCTGCTCGCCCAGCAGCTTCCGTTAGCGGATGTTGTAAAAGGCGCCCGGGTTTACGTCGACGGCTTCTATCAATTTA
CACCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGGAGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAAAGGCATCAGCATGTGCCGGAACTTGCCTGCATAG
AGTCGCAATACGATGTGCGTCCCGCTGCGGCGTATACCGGCCGGCAGGAAGCGTTTACCGTCATACAGGCGCAAAACAGA
AGAGCTGAGCTTGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACCGGGATATGGCGAT
TCTCATCCGCCAGCCGGAAGATTATAAAGATTTGCTCAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATAGAATTCATCCGTTCAAGTTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGACCAGCCGGTGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTATTGTATCGCCTACGGAATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAGCAAATGCTGAATGAGACGAGAGAATGG
ATGGCCGCGCCCCTCGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCAGGCCGAAGAGGCCGGAAACATGATTGAAG
CGCAGCAGCACGGCCAGGCATGGGACGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGATTGAAACGGGAACGGAATCACTTCATTTTTCTTTAATTCCCCCTGCGCTTGACCA
AGTGTTTGTAGGGAATATGGACCTGTCAAGAATGTACGGCACGTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCCGATGATGACCGGGAGTGGCTGAGAGCGGTCGGAGTGGAGCTGTCT
TCGGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GTCATATCCGATTGCCGATGCGGAAGGAAAAACGCTTCTGCCCTCAATTGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ACCATCAGGAAAAACTGTCGGCTGCTGATCCCGAGCAGGTAAGTGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTATGAAATCAGTGATGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCGGCGCGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCAAAGC
GTCTTGAACGTCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCCCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCCGATATCGG
ACAGCTGTTTCACTCCAGCCTCAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCTGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGATCGGCCTTGAACTCGGTTTCGGCGGAAACGGTCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTTAGAATCGTG
GATTACAAATCAAGCGACAAGGGGCTTGATCTGGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACGTACCTTGA
CTTATCGATTACACATTCAGCGGATTGGCTCGGCATGAAAGCGACACCCGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCAATCAGCCTGATGGACACGACTTTGGAGGAAGGACGTTCAAATATCATTAATGCCGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGATTCAGCCGCAGTCAGCGAACAGGATTTTCATCTCCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCTCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(310-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.276

100

0.833

Multiple sequence alignment