Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   WA044_RS16750 Genome accession   NZ_CP151554
Coordinates   3374295..3375680 (-) Length   461 a.a.
NCBI ID   WP_058906395.1    Uniprot ID   -
Organism   Bacillus velezensis strain E69     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3369295..3380680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA044_RS16715 (WA044_16715) flgL 3369470..3370387 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  WA044_RS16720 (WA044_16720) flgK 3370399..3371916 (-) 1518 WP_003151395.1 flagellar hook-associated protein FlgK -
  WA044_RS16725 (WA044_16725) - 3371932..3372414 (-) 483 WP_058906392.1 flagellar protein FlgN -
  WA044_RS16730 (WA044_16730) flgM 3372429..3372695 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  WA044_RS16735 (WA044_16735) - 3372765..3373184 (-) 420 WP_058906393.1 TIGR03826 family flagellar region protein -
  WA044_RS16740 (WA044_16740) comFC 3373258..3373947 (-) 690 WP_196479391.1 ComF family protein Machinery gene
  WA044_RS16745 (WA044_16745) - 3373953..3374237 (-) 285 WP_003151389.1 competence protein ComFB -
  WA044_RS16750 (WA044_16750) comFA 3374295..3375680 (-) 1386 WP_058906395.1 DEAD/DEAH box helicase Machinery gene
  WA044_RS16755 (WA044_16755) - 3375788..3376636 (-) 849 WP_058906396.1 DegV family protein -
  WA044_RS16760 (WA044_16760) degU 3376734..3377423 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  WA044_RS16765 (WA044_16765) degS 3377500..3378663 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  WA044_RS16770 (WA044_16770) - 3378886..3379533 (+) 648 WP_003151384.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52471.91 Da        Isoelectric Point: 10.0900

>NTDB_id=979147 WA044_RS16750 WP_058906395.1 3374295..3375680(-) (comFA) [Bacillus velezensis strain E69]
MAEGASEFSEVRDFFYGRHLLRSEIPFSDHCIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIKF
ALNQGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALIYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPHFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVLFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=979147 WA044_RS16750 WP_058906395.1 3374295..3375680(-) (comFA) [Bacillus velezensis strain E69]
ATGGCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCATTGCATCAAGCGTTTTATAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAGAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCAAGTTT
GCCCTGAATCAAGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTTGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTATTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAATGCTGAAGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCCCTGCCGG
AGCCGCATTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGATCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCCTGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623

Multiple sequence alignment