Detailed information NTG0979131

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: WA044_RS12295
• Genome accession: NZ_CP151554
• Coordinates: 2524742..2525356 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain E69
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2519742..2530356

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WA044_RS12265 (WA044_12265)	rpsT	2520042..2520308 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
WA044_RS12270 (WA044_12270)	holA	2520325..2521366 (-)	1042	Protein_2369	DNA polymerase III subunit delta	-
WA044_RS12275 (WA044_12275)	-	2521406..2521560 (-)	155	Protein_2370	hypothetical protein	-
WA044_RS12280 (WA044_12280)	-	2521601..2521735 (+)	135	WP_003152870.1	YqzM family protein	-
WA044_RS12285 (WA044_12285)	comEC	2521754..2524105 (-)	2352	WP_058906194.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
WA044_RS12290 (WA044_12290)	-	2524106..2524675 (-)	570	WP_003152868.1	ComE operon protein 2	-
WA044_RS12295 (WA044_12295)	comEA	2524742..2525356 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
WA044_RS12300 (WA044_12300)	comER	2525415..2526236 (+)	822	WP_014305449.1	late competence protein ComER	-
WA044_RS12305 (WA044_12305)	-	2526305..2527042 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
WA044_RS12310 (WA044_12310)	rsfS	2527039..2527395 (-)	357	WP_003152864.1	ribosome silencing factor	-
WA044_RS12315 (WA044_12315)	yqeK	2527392..2527973 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
WA044_RS12320 (WA044_12320)	-	2527963..2528532 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
WA044_RS12325 (WA044_12325)	yhbY	2528543..2528833 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
WA044_RS12330 (WA044_12330)	aroE	2528827..2529669 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=979131 WA044_RS12295 WP_003152867.1 2524742..2525356(-) (comEA) [Bacillus velezensis strain E69]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=979131 WA044_RS12295 WP_003152867.1 2524742..2525356(-) (comEA) [Bacillus velezensis strain E69]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363