Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AADV58_RS01955 Genome accession   NZ_CP151406
Coordinates   413052..414506 (+) Length   484 a.a.
NCBI ID   WP_028993836.1    Uniprot ID   -
Organism   Azonexus hydrophilus strain NCP973     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408052..419506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADV58_RS01925 (AADV58_01925) - 408289..409707 (-) 1419 WP_341743938.1 sodium:solute symporter family protein -
  AADV58_RS01930 (AADV58_01930) - 409750..410430 (-) 681 WP_341743939.1 pseudouridine synthase -
  AADV58_RS01935 (AADV58_01935) - 410431..410634 (-) 204 WP_028993840.1 hypothetical protein -
  AADV58_RS01940 (AADV58_01940) recA 410847..411869 (+) 1023 WP_028993839.1 recombinase RecA Machinery gene
  AADV58_RS01945 (AADV58_01945) recX 411862..412296 (+) 435 WP_341743940.1 recombination regulator RecX -
  AADV58_RS01950 (AADV58_01950) lptB 412293..413027 (+) 735 WP_028993837.1 LPS export ABC transporter ATP-binding protein -
  AADV58_RS01955 (AADV58_01955) rpoN/rpoN1 413052..414506 (+) 1455 WP_028993836.1 RNA polymerase factor sigma-54 Machinery gene
  AADV58_RS01960 (AADV58_01960) raiA 414521..414844 (+) 324 WP_028993835.1 ribosome-associated translation inhibitor RaiA -
  AADV58_RS01965 (AADV58_01965) ptsN 414896..415345 (+) 450 WP_028993834.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AADV58_RS01970 (AADV58_01970) hprK 415354..416289 (+) 936 WP_028993833.1 HPr(Ser) kinase/phosphatase -
  AADV58_RS01975 (AADV58_01975) - 416311..417201 (-) 891 WP_341743941.1 adenylate/guanylate cyclase domain-containing protein -
  AADV58_RS01980 (AADV58_01980) ttcA 417264..418133 (-) 870 WP_028993831.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  AADV58_RS01985 (AADV58_01985) - 418243..419403 (+) 1161 WP_281985655.1 SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 54316.38 Da        Isoelectric Point: 4.8732

>NTDB_id=976434 AADV58_RS01955 WP_028993836.1 413052..414506(+) (rpoN/rpoN1) [Azonexus hydrophilus strain NCP973]
MKPALQLKLSQHLALTPQLQQSIKLLQLSTVEMQQEIERYLLENPMLERDDDGPGESFAGAQQFDSPDKPVSEPAPASES
REDAQGEPPMTPGDVDDDRWSSDAGTFTGAGRDEEDDNDPQDAHASSVSLREHLGWQLGMTQLSDRDRSLVRFLIEALSD
DGYLATPLAELLETLPPEYEVELEELEIALRHIQNFDPTGIGARDLRECLLLQLQVEEDCAERTLALTIADKHLELLAAR
DFVKIRRLTGCDDEILRGAHELITSLNPRPAAGYAQDEARYITPDVIVKKLKGKWTAYINPDAYPRLRVNRLYADILARQ
RRGNGNLSGQLQEARWLIKNIQQRFDTIHRVTQAIVDRQRQFFEHGEVAMRPLVLREIADLLGLHESTISRVTSQKYMAT
PRGIFELKYFFGSHVSTDTGGACSATAIRALIKQLISAEDGKKPLSDSQLSEILGQQGIVVARRTVAKYRESLNIPPVNL
RKTL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=976434 AADV58_RS01955 WP_028993836.1 413052..414506(+) (rpoN/rpoN1) [Azonexus hydrophilus strain NCP973]
ATGAAACCGGCACTTCAGCTCAAGCTTTCGCAGCATCTGGCCCTGACGCCGCAATTACAGCAGTCGATCAAGCTGCTGCA
GTTGTCGACCGTCGAGATGCAGCAGGAAATAGAACGCTACCTGCTGGAAAACCCGATGCTCGAGCGCGACGACGACGGAC
CGGGCGAGTCCTTCGCCGGTGCCCAGCAGTTCGATTCGCCCGACAAGCCGGTCAGCGAACCCGCACCGGCGAGCGAGTCG
CGTGAGGATGCCCAGGGTGAGCCGCCGATGACGCCCGGTGACGTCGACGACGATCGCTGGTCCAGCGATGCCGGCACCTT
CACCGGCGCCGGCCGCGACGAGGAAGACGACAACGATCCGCAGGACGCCCACGCTTCCAGCGTCAGCCTGCGCGAGCACC
TCGGCTGGCAACTGGGGATGACCCAGTTGAGCGATCGCGATCGCAGTCTGGTCCGTTTCCTGATCGAAGCACTCAGCGAC
GACGGCTATCTCGCCACGCCGCTGGCAGAACTGCTGGAAACCCTGCCGCCCGAGTACGAGGTCGAGCTCGAGGAGCTCGA
AATCGCCCTGCGCCATATCCAGAATTTCGATCCGACCGGGATCGGTGCCCGCGACCTGCGCGAATGTCTGCTGCTGCAAC
TGCAGGTCGAAGAGGATTGCGCCGAGCGCACGCTGGCCCTGACCATTGCCGACAAGCACCTGGAATTGCTGGCTGCCCGC
GATTTCGTCAAGATCCGCCGCCTGACCGGCTGCGATGACGAAATCCTGCGCGGCGCGCACGAGCTGATCACCAGCCTCAA
TCCGCGGCCGGCCGCCGGTTACGCACAGGACGAAGCGCGCTACATCACGCCCGACGTGATCGTGAAGAAGCTCAAGGGTA
AATGGACCGCCTACATCAACCCGGATGCTTACCCGCGCCTGCGCGTCAATCGCCTGTACGCCGACATCCTTGCCCGCCAG
CGTCGCGGCAATGGCAACCTGAGCGGCCAGTTGCAGGAAGCCCGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGATAC
CATCCACCGCGTCACCCAGGCCATCGTGGACCGCCAGCGCCAGTTCTTCGAACATGGCGAAGTGGCGATGCGGCCACTGG
TGCTGCGCGAGATCGCCGACTTGCTCGGCCTGCACGAATCGACGATTTCCCGCGTTACCAGCCAGAAATACATGGCCACG
CCGCGCGGCATCTTCGAACTGAAATACTTCTTCGGCAGCCATGTGTCCACCGATACTGGTGGTGCCTGTTCTGCCACGGC
CATTCGTGCCCTGATCAAGCAATTGATTTCGGCGGAAGATGGCAAGAAGCCCTTGTCGGATAGTCAATTATCCGAAATAC
TAGGCCAGCAGGGAATTGTCGTTGCCCGACGCACGGTTGCCAAATACCGTGAATCGCTCAACATCCCACCGGTCAATTTA
CGCAAGACCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(121-313)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-482)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.171

100

0.593

Multiple sequence alignment