Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ABWW09_RS09505 Genome accession   NZ_AP031332
Coordinates   1747170..1748816 (-) Length   548 a.a.
NCBI ID   WP_353300272.1    Uniprot ID   -
Organism   Thermus thermophilus strain TF27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1742170..1753816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW09_RS09475 (TthTF27_18700) - 1742741..1743412 (-) 672 WP_353300268.1 response regulator transcription factor -
  ABWW09_RS09480 (TthTF27_18710) - 1743472..1744599 (-) 1128 WP_353300269.1 alanine--tRNA ligase-related protein -
  ABWW09_RS09485 (TthTF27_18720) - 1744619..1744816 (+) 198 WP_014630250.1 hypothetical protein -
  ABWW09_RS09490 (TthTF27_18730) - 1744813..1745544 (+) 732 WP_353300270.1 pseudouridine synthase -
  ABWW09_RS09495 (TthTF27_18740) - 1745512..1745979 (-) 468 WP_014630252.1 hypothetical protein -
  ABWW09_RS09500 (TthTF27_18750) pilC 1745976..1747166 (-) 1191 WP_353300271.1 type II secretion system F family protein Machinery gene
  ABWW09_RS09505 (TthTF27_18760) pilB/pilB1 1747170..1748816 (-) 1647 WP_353300272.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ABWW09_RS09510 (TthTF27_18770) - 1748794..1749981 (-) 1188 WP_244344461.1 hypothetical protein -
  ABWW09_RS09515 (TthTF27_18780) - 1749991..1750830 (-) 840 WP_353300273.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWW09_RS09520 (TthTF27_18790) - 1750827..1751573 (-) 747 WP_353300274.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWW09_RS09525 (TthTF27_18800) - 1751570..1751962 (-) 393 WP_353300275.1 hypothetical protein -
  ABWW09_RS09530 (TthTF27_18810) - 1751966..1752418 (-) 453 WP_124105084.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWW09_RS09535 (TthTF27_18820) - 1752428..1752856 (-) 429 WP_014630259.1 type II secretion system protein -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 61201.78 Da        Isoelectric Point: 6.0720

>NTDB_id=97625 ABWW09_RS09505 WP_353300272.1 1747170..1748816(-) (pilB/pilB1) [Thermus thermophilus strain TF27]
MARPKLGELLLDLGYITEDQLKAALEEQERTGDLLGQILLRRGYIREEDLVRALADQQRAPLIHPAQTPLDPQALRLLDP
RFAREKRVLPFKVEGNRLHVAMAHPSDLALLDELRFRTGKEIVPYLAPDREILEVLDRLLAEEARPREEARAREEAEAVD
LTLEAMPAVRLAQALLERAIALSASDLHLDPQETHLQVRARIDGVMQDLERLPKDLEAPLAARYKVLAGMDIAERRRPQD
GHFTFSFEGRRYEVRVASVGTLHGEKLTLRIIYPTGVRLGLTELGMLPEELDRFQKLLKRPYGILFVTGPTGSGKTTTLY
AALEQLYTKEKTFVTIEDPVEFPLEGVVQIPVQPKIGLTFAEALRTVLRLDPDVILVGEVRDAETLDTALRAALTGHLVL
ATLHANDAVAALTRLVEMGAERPLLAATLIGTVAQRLVRRVCPQCAKPMPVPEEARLYLGERAPEVEKRGVGCPYCRGTG
YRGRIGVYEVYAPDREALSRFAQGAGEEALRERARALGHRDLWEVGLLQVRAGMTTTGELLRVLGLWE

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=97625 ABWW09_RS09505 WP_353300272.1 1747170..1748816(-) (pilB/pilB1) [Thermus thermophilus strain TF27]
TTGGCCCGGCCCAAGCTCGGTGAACTTCTTTTGGACCTCGGCTACATCACCGAGGACCAGCTGAAGGCCGCCCTGGAGGA
GCAGGAGCGCACCGGGGACCTCCTGGGCCAGATCCTCCTCCGGCGGGGCTACATCCGGGAGGAGGACCTGGTGCGGGCCC
TGGCCGACCAGCAGCGGGCTCCCTTGATCCACCCCGCCCAGACCCCCCTGGACCCCCAGGCCCTGCGCCTTTTGGACCCC
AGGTTCGCCCGGGAGAAGCGGGTTCTGCCCTTCAAGGTGGAGGGCAACCGCCTCCACGTGGCCATGGCCCACCCCTCGGA
CCTGGCCCTCTTGGACGAGCTCCGCTTCCGCACCGGGAAGGAGATCGTCCCCTACCTGGCCCCGGACCGGGAGATCCTGG
AGGTCCTGGACCGCCTCCTGGCGGAGGAGGCCCGGCCCCGGGAGGAGGCTCGGGCCCGGGAGGAGGCCGAGGCGGTGGAC
CTCACCCTCGAGGCCATGCCCGCCGTCCGCCTGGCCCAGGCCCTTCTGGAGCGGGCCATCGCCCTCTCGGCCAGCGACCT
CCACCTGGACCCTCAGGAAACCCACCTCCAGGTCCGGGCTCGCATTGACGGGGTGATGCAGGACCTGGAGCGCCTTCCCA
AGGACCTGGAGGCGCCCCTGGCCGCCCGCTACAAGGTCCTCGCGGGCATGGACATCGCCGAGAGGCGCCGTCCCCAGGAC
GGCCACTTCACCTTCTCCTTTGAGGGCAGGCGCTACGAGGTGCGGGTGGCCTCGGTAGGCACGCTCCACGGGGAGAAGCT
CACCCTCCGCATCATCTACCCCACGGGGGTGCGGCTCGGCCTCACCGAGCTCGGCATGCTTCCCGAGGAGCTGGACCGCT
TCCAGAAGCTCCTCAAGAGGCCCTACGGCATCCTCTTCGTCACCGGCCCCACGGGAAGCGGCAAGACCACTACCCTCTAC
GCCGCTTTGGAACAGCTTTACACCAAGGAGAAGACCTTCGTCACCATTGAGGACCCGGTGGAGTTTCCCCTCGAGGGGGT
GGTCCAGATCCCCGTCCAGCCCAAGATCGGCCTCACCTTCGCCGAGGCCTTGCGGACCGTTCTCCGCCTGGACCCCGACG
TGATTTTGGTGGGGGAGGTGCGGGACGCCGAGACCCTGGACACCGCCCTTAGGGCCGCCCTCACGGGGCACCTCGTCCTC
GCCACCCTCCACGCCAACGACGCCGTGGCCGCCCTCACCCGTCTCGTGGAGATGGGGGCGGAAAGGCCCCTCCTCGCCGC
CACCCTCATCGGCACCGTGGCCCAGCGCCTGGTGCGCCGGGTCTGCCCCCAGTGCGCCAAGCCCATGCCCGTCCCCGAGG
AGGCCCGGCTCTACCTGGGGGAGCGGGCCCCCGAGGTGGAGAAGCGGGGGGTGGGGTGCCCCTACTGCCGGGGGACGGGC
TACCGGGGCCGGATCGGGGTCTACGAGGTCTACGCCCCGGACCGGGAGGCCCTCTCCCGCTTCGCCCAGGGAGCGGGGGA
GGAGGCCCTAAGGGAGCGGGCCCGGGCCCTGGGGCACCGGGACCTCTGGGAGGTGGGCCTCCTTCAGGTGCGGGCGGGGA
TGACCACCACAGGGGAGCTCCTTCGGGTCCTCGGGCTTTGGGAGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-144)

Type II/IV secretion system protein (T2SSE)

(175-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.75

100

0.396
  pilF Thermus thermophilus HB27

38.739

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.657

100

0.389
  pilB Acinetobacter baylyi ADP1

37.255

100

0.381
  pilB Vibrio parahaemolyticus RIMD 2210633

35.284

100

0.363
  pilB Vibrio campbellii strain DS40M4

35.106

100

0.361

Multiple sequence alignment