Detailed information NTG0974896

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: AABL52_RS21150
• Genome accession: NZ_CP151108
• Coordinates: 4125408..4129133 (-)
• Length: 1241 a.a.
• NCBI ID: WP_341518333.1
• Uniprot ID: -
• Organism: Bacillus paramobilis strain IMGN7
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4120408..4134133

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AABL52_RS21100 (AABL52_21100)	-	4121333..4121617 (+)	285	WP_076535967.1	hypothetical protein	-
AABL52_RS21105 (AABL52_21105)	-	4121651..4122550 (-)	900	WP_176546283.1	fumarylacetoacetate hydrolase family protein	-
AABL52_RS21110 (AABL52_21110)	-	4122797..4122976 (+)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
AABL52_RS21115 (AABL52_21115)	gerPA	4123073..4123294 (+)	222	WP_001111187.1	spore germination protein GerPA	-
AABL52_RS21120 (AABL52_21120)	gerPB	4123309..4123515 (+)	207	WP_001012513.1	spore germination protein GerPB	-
AABL52_RS21125 (AABL52_21125)	gerPC	4123584..4124198 (+)	615	WP_071721606.1	spore germination protein GerPC	-
AABL52_RS21130 (AABL52_21130)	gerPD	4124205..4124399 (+)	195	WP_001052807.1	spore germination protein GerPD	-
AABL52_RS21135 (AABL52_21135)	-	4124415..4124801 (+)	387	WP_088095504.1	spore germination protein GerPE	-
AABL52_RS21140 (AABL52_21140)	gerPF	4124844..4125059 (+)	216	WP_001141566.1	spore germination protein GerPF	-
AABL52_RS21145 (AABL52_21145)	-	4125108..4125395 (-)	288	WP_341518332.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
AABL52_RS21150 (AABL52_21150)	addA	4125408..4129133 (-)	3726	WP_341518333.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
AABL52_RS21155 (AABL52_21155)	addB	4129130..4132645 (-)	3516	WP_341518334.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
AABL52_RS21160 (AABL52_21160)	lepB	4132762..4133325 (-)	564	WP_048545235.1	signal peptidase I	-
AABL52_RS21165 (AABL52_21165)	-	4133382..4133975 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142547.91 Da
• Isoelectric Point: 4.9154

>NTDB_id=974896 AABL52_RS21150 WP_341518333.1 4125408..4129133(-) (addA) [Bacillus paramobilis strain IMGN7]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEAEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSLAARKSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEAIEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYSASWK
VEVVDGSTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYDEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKMRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQISGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGQSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=974896 AABL52_RS21150 WP_341518333.1 4125408..4129133(-) (addA) [Bacillus paramobilis strain IMGN7]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGGTCGCAGCATGTAAGAAAGCAACTTAGCTTATTAAATAAAGC
TTCTATTTCAACGATTCACTCCTTTTGTTTACAAGTAATTAGAGGATACTACTACATGCTTGATGTTGATCCTCGTTTTC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTATTAGATGACATATTAGAAGCAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGATCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCTTGCAAGCTGATGCAGTTTTACT
TGGAACGTTATCATTAGCTGCTCGTAAATCGTGGACAAGTGTGTACGAAGCGATGCAAAACGTATCGTGGCAAACATTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAGATGAAGCCATCAGCAGTAGCGCTCCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCCATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGGGACGTAAAACAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGTTTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTATGATGCAGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACGGAAGAAGAAGTGCAAGACGGTGAAGAAGGTGCAGAAGTAGAAAAAGCGCAACTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGATAGAAGTAAATATTATGATGAACGTATTCCGCGTAATCGATAAT
CCGATGCAAGATATTCCACTTGCCGCAGTGCTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGTTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGGGCACCGCTTGAGGAAGAACAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAGTCTCTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGATTAGAGTTCCCAGTCGTGTTTGTTGCGGGGCTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGTATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCCGAAGAAATGCGCGTATTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATCGGAACGGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCGCCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAGTATTCCAGGTGAGATTTATGGATATAGTGCGAGCTGGAAA
GTTGAAGTTGTAGACGGTAGCACGTTACTTGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGATG
AAGCAACATCTCACCGCGCGAAACAATCTGTTACGGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAAATGCGTGCGCCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGATTAACATACGCTGAAAGAGG
TACGGCAGTTCATGCTGTCATGCAACATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTAGTG
GCATGGTTAATAAAGAACTATTAACATTCGAACAAGCCGAAGAAATAGCAATCGAAAAAGTCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTATTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGACAGAGTGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GTATCACTTTAATCGACTTTAAAACAGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTGTATGCAAAGGCACTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTGAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1061-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535